LOCUS       sequence-315577-        5288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0021:Dendra
FEATURES             Location/Qualifiers
     source          1..5288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1232..1255
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1512..2312
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2437..3025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2926..2945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcaaagagt gctgctggca agcaagattc accgcgcaac
      121 agtgactcaa gcggacctgc actacgtcgg gtccataacg atcgacgcgg aactgatggc
      181 agccgcggac atcgtcgagg gtgaacaggt gcacgtcgtg gacatcacga gcggggcacg
      241 actggtcacc tacgccatca ccggcacccc ggggtcgggt gtgatcggca tcaacggcgc
      301 tgccgcccat ctcatttcgc caggaaacct ggtgatcatc atgtcgttcg tacatctcga
      361 cgaagccgaa cgtgccgatc accgggcaaa cgtcgtgcac gtcgacgccg acaaccacat
      421 cgtcaccatc ggatccgacc cggcccagcc ggtaccaggg gcggccgatc agctggtggg
      481 acgggtgaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga
      541 catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg
      601 ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg
      661 cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt
      721 gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata
      781 ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat
      841 ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc
      901 gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca
      961 cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg
     1021 ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga
     1081 cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt
     1141 caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga
     1201 aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt
     1261 taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg
     1321 atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     1381 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag
     1441 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
     1501 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
     1561 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
     1621 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
     1681 tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
     1741 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
     1801 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
     1861 ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa
     1921 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
     1981 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     2041 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     2101 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     2161 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     2221 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg
     2281 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     2341 gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg
     2401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2581 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2821 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     2881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3061 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3121 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3181 cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg
     3241 cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg
     3301 ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt
     3361 ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag
     3421 gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac
     3481 cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg
     3541 ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag
     3601 ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac
     3661 cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag
     3721 cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag
     3781 ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca
     3841 tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg
     3901 gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct
     3961 ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg
     4021 aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg
     4081 gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg
     4141 gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac
     4201 aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg
     4261 tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag
     4321 ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg
     4381 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
     4441 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
     4501 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
     4561 gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc
     4621 ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag
     4681 cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc
     4741 gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac
     4801 acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc
     4861 gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag
     4921 gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc
     4981 gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag
     5041 cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt tgatcgggga
     5101 cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg atccgcgacg
     5161 tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc ccggcgttga
     5221 tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc gggagaactc
     5281 cctatcag
//
