LOCUS       sequence-315578-        5486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0240:Dendra
FEATURES             Location/Qualifiers
     source          1..5486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1430..1453
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1710..2510
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2635..3223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3124..3143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcccccga cgttcgggac atgcgggacg cgcgtcgcaa
      121 agacatgtcg cccatacaac cgccctcgaa ccgatccccc ggccaacgga accaagggcc
      181 gcgcccggca gcggtgcgcc cgcgggatcc ccaggctccc gcccgcggcg cagcgcgcgg
      241 tccccagcga cagttcgacg accgcccggt cgagtactgg cccaccgcgg cgatccgcgc
      301 ggcactggag accgacgatc tgacggtgtg gcagcgcatc gtcgctgcga tcaaacggga
      361 cccgtacggc cgcaccgccc gtcaggtcga ggaggtcctg gagaccgccc ggccctacgg
      421 cgtctcgaaa gccctgtcgg aagttctcgt gcgcacccgc gaccacctcg aggcgtctga
      481 acgcgccgag gtggccaagc agatccaggc catgctgcgc cggtcgaaac tcgcgccgcc
      541 ggaattcgcc tcgcgcgtcg gggtgtccaa cgaagagttc gcgacgtatc tggagggcag
      601 tgtgagcccc cgtgcgtcgc tgctgctgcg gatgcagcgg ttgtcggacc ggttcgcgaa
      661 gatcgccgcg cagcgcagcg gcaagaagct tggtgcggcc gcgggcaaca cgcctggcat
      721 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
      781 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
      841 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
      901 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
      961 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
     1021 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     1081 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     1141 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     1201 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     1261 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     1321 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     1381 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     1441 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     1501 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     1561 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     1621 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     1681 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     1741 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     1801 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     1861 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     1921 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     1981 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     2041 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     2101 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     2161 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     2221 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     2281 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     2341 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     2401 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     2461 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     2521 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     2581 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2641 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2701 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2761 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2821 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2881 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2941 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3001 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     3061 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3241 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3301 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3361 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     3421 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     3481 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     3541 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     3601 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     3661 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     3721 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     3781 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     3841 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     3901 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     3961 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     4021 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     4081 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     4141 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     4201 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     4261 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     4321 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     4381 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     4441 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     4501 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     4561 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     4621 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     4681 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     4741 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     4801 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     4861 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     4921 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     4981 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     5041 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     5101 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     5161 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     5221 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     5281 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     5341 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     5401 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     5461 tgacgagcgg gagaactccc tatcag
//
