LOCUS       sequence-315580-        6134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0385:Dendra
FEATURES             Location/Qualifiers
     source          1..6134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2078..2101
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2358..3158
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3283..3871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3772..3791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcgagtta tggcacccgc ggtgatgtcg aaccggctgt
      121 tgcggtggct cgtgagctac gcagacgagg ccatgatgtg tgcatcgccg tcccacctga
      181 cctcgtgcgg ttcgccgagg acgccgggct ctcggccact tcgtacggca tggaggtcga
      241 gccgcagttg gacgcgtacc gcgacatatg gacttcgtgg gcacgtaaac tctggcgcct
      301 gcaggatttc gtgaacctct gcagagaggc actgggcgtg acgatctctc agtgggagga
      361 catcggcaag actctggtga cgctcgccga cggggcagac ctgctcacga ccggggtagg
      421 tttcgagcag cctgccgcca acgtcgcgga gtattacggt attccgctga ttgcgctgca
      481 cacgttccct tggcgtccga acggccgact tgttccggca ataccacctt tactcacacg
      541 ctcggctatg aacgtctacg actggctggg ctggcgcctg acgaagcaag ccgaggacgc
      601 acagcggcgc gagttgggct tgccgatggc gactagtgcc gcccggcagc ggatggcgga
      661 acggggcgtt ctggagatcc aggcctatga cgcgatctcc ttccccggtc tcgccgacga
      721 gtgggcacgg tatggcgcca gacgaccctt cgtcggcact ctgacgatgg agttggcgac
      781 cgcggacgac agtgaggtca cgtcgtggat cgcgcacgga acgccgccga tctgcttcgg
      841 atttggcagt atcccggtcg aatctcccgc cgagacaatc gacatgatcg cgtcggcatg
      901 cgcggagttg ggtgagcggg cgctggtgtg tgccggcggg acggacttcg gtgattcacc
      961 caggttcgag cacgtgaagg tcgcgggaac cgtcaactat gcgacggtgt tcccggtctg
     1021 ccgtgctgtg gtccatcacg gcggctcggg aacgacagcg gcgagccttc gtgccggagt
     1081 gcccacgttg actctgtgga cagctggtga tcagccgttc tggggcgcgc agctgaagaa
     1141 gttgaaagta ggtgcatcgc gccgtttctc ggccacgacg cgcgataccc tcgtgtcgga
     1201 cctgcgccgg atcctctctc ccgaatatgc cgtcaaggcg cgtgaggtcg ccacccggat
     1261 gacgaatccg gctgaaagtg tcagccgagc agccgatctc atcgaacaga acctcgatcc
     1321 cacacgccgc tcgaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1381 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1441 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1501 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1561 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     1621 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     1681 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     1741 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     1801 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     1861 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     1921 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     1981 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     2041 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     2101 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     2161 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     2221 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2281 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2341 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2401 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2461 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2521 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     2581 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     2641 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     2701 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     2761 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     2821 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     2881 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     2941 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     3001 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3061 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3121 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     3181 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3241 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3661 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     3721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4021 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     4081 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     4141 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     4201 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4261 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4321 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4381 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4441 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4501 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4561 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4621 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     4681 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     4741 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     4801 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     4861 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     4921 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     4981 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     5041 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     5101 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     5161 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     5221 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5281 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5341 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5401 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5461 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
     5521 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
     5581 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
     5641 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
     5701 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
     5761 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
     5821 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
     5881 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
     5941 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
     6001 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
     6061 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
     6121 gaactcccta tcag
//
