LOCUS       sequence-315582-        5504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0635:Dendra
FEATURES             Location/Qualifiers
     source          1..5504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1448..1471
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1728..2528
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2653..3241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3142..3161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcggccgt ggcactggcg tgcgcaccga gcgcggcagc
      121 cgtggagaac ccgtggttcg cgcgatcggt cggcactgcc acccaggtga tttccgttgt
      181 gggcgtgggt ggttcgaagg ccaagatgga tgtctaccag cgcaccgcgg caggctggca
      241 gcccgtgggc gtaggcatcg aagcctggat cggcgccaac gggatggccc cgcagaccca
      301 cgacggcgag atgaagacgc cgatgggcat cttcacgctc gacttcgcgt tcgggaccgc
      361 gccgaatccc ggtggcgggc tgaaatatgt gcaggtgggc ccggaccact ggtgggacgg
      421 cgacatgaag agccccacct acaacaccat gcaggtgtgc aagaaggcac agtgcccgtt
      481 cgacaccgac ccccgcagcg gcaccgagaa tctgaacatc ccgcagtacc ggcatgccgt
      541 ggtgatgggc gtgaacaagg ccagggtgcc gggcaacggc ggagccttct tcgtgcacaa
      601 caccgacggc gggccgaccg cgggatgtgt ggccatcgac gacgcgacgc tggtacagat
      661 catccgctgg ttgcggccgg gcgcgctgat cgcggtggcg aagaagcttg gtgcggccgc
      721 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
      781 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
      841 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
      901 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
      961 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1021 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     1081 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     1141 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     1201 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     1261 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     1321 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     1381 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     1441 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     1501 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     1561 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     1621 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     1681 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     1741 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     1801 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     1861 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     1921 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     1981 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     2041 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     2101 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     2161 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     2221 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     2281 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     2341 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     2401 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     2461 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     2521 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     2581 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     2641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2761 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3061 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3361 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     3421 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     3481 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     3541 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     3601 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     3661 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     3721 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     3781 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     3841 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     3901 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     3961 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4021 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     4081 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     4141 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     4201 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     4261 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     4321 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     4381 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     4441 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     4501 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     4561 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     4621 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     4681 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     4741 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     4801 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     4861 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     4921 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     4981 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     5041 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     5101 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     5161 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     5221 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     5281 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     5341 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     5401 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     5461 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
