LOCUS       sequence-315585-        6341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0035:Dendra
FEATURES             Location/Qualifiers
     source          1..6341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2285..2308
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2565..3365
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3490..4078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3979..3998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaaggcggg cgatgtcatg ggtctggtag accgcatcga
      121 acgcaagctc gagtccacgg tcggagacgc cttcgcgcgg gtcttcggcg ggtcgatcgt
      181 gccgcaggaa gtcgaggtgg cgctgcggcg ggaagccgag acccgcgcac gcaccgtggc
      241 aggtggccaa gttttggcac cgaacgacta cgtaattacc ctcagtggaa ctgactatca
      301 gaaggtaagg gccgatactg acctgacctc gaccacgttc gccaagcatc tgaagggata
      361 catccatgat cagggttggc aaacgtatgg tgaggtggtc gtcagattcg agcaatcacc
      421 gaatttgcac actggacaat tccgcgcgcg tggcgcggtc aaccccgatt cgaccgctgg
      481 cgaatccgcg cgagctccac gggaccatgc gttcaccgca gaatcaggag aaccacctat
      541 gaccgataac ccgaattacc gaggcggcca gggacccgcg cggcccggcg acgagtacta
      601 cgacgagcgc tacggacgcc aggacgatcc gcgtggcggg cagtacccgc cggagcaggg
      661 tggctatccc ccgcaggacc agggcggcta cccgccccgc ggcggcggtt atcccgacca
      721 gggcggctat ccggatcagg gtggataccc cgaccagggt ggttacccgg accagggcgg
      781 atacccgccg cagtcctatg agcagcgccc gcccgcgggt tacggcccgc cgccgcaggg
      841 cggcggctac gaccgtggct acggccagcc ggccggcggc tacccgcccg caggccctcc
      901 ggccggaccg ccccccggcg gccagcccgg ttacggcggt gccgattacg actacggcag
      961 gcaggccccg cgccacgacg actacggccg tccggatccg cgccagggct atcccgagca
     1021 gggtggctac cccgaacagg gcggctacgg cggccaggcc tacggccgtc cggactacgg
     1081 ccagcccgat tacggtcagc agcccggccg cggctacccc gagcagggcg gttaccccga
     1141 gcagggtgga tacggcgagc ccgccggtcg tgactacgag tacggcggcg attacggcgc
     1201 tccggccggt cagggcggat acggcggcgg ttacggcgcc gactatccgg ccggtggcac
     1261 gacggtgacg ctgcagctcg acgacggcag cggccgcacc taccagctgc gtgagggcgc
     1321 caacgtgatc ggccgtggcc aggacgcaca gttccggctg cccgacaccg gcgtgtcgcg
     1381 tcggcacctc gagatccgct gggacggcca ggtggcgttg ctctccgatc tgaactccac
     1441 caacggcacc acggtgaaca acgcaccggt ccaggagtgg cagctggcgg acggcgacgt
     1501 catccggctg ggccactccg agatcatcgt gcgcgtgcac aagcttggtg cggccgcggg
     1561 caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg
     1621 caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg
     1681 gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat
     1741 cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc
     1801 tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga
     1861 agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca
     1921 gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac
     1981 cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa
     2041 catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac
     2101 gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga
     2161 aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg
     2221 ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt
     2281 ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg
     2341 ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact
     2401 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
     2461 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
     2521 ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg
     2581 agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc
     2641 ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc
     2701 tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga
     2761 cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc
     2821 ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc
     2881 gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg
     2941 atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg
     3001 atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg
     3061 agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca
     3121 agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg
     3181 acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc
     3241 ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt
     3301 tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga
     3361 ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     3421 ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa
     3481 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3541 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3601 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3661 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3721 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3781 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3841 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3901 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3961 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4021 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4081 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4141 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4201 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc
     4261 ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca
     4321 gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga
     4381 ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa
     4441 tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca
     4501 tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc
     4561 acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca
     4621 gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg
     4681 accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg
     4741 aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc
     4801 cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg
     4861 aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg
     4921 tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag
     4981 aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc
     5041 gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca
     5101 gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt
     5161 gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt
     5221 aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca
     5281 gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat
     5341 gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc
     5401 gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg
     5461 atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc
     5521 cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg
     5581 aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac
     5641 cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg
     5701 ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg
     5761 gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg
     5821 acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac
     5881 cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag
     5941 acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt
     6001 cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg
     6061 agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga
     6121 cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg
     6181 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
     6241 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
     6301 tgtgagaagg gtctctgacg agcgggagaa ctccctatca g
//
