LOCUS       sequence-315586-        5600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0252:Dendra
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1544..1567
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1824..2624
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2749..3337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3238..3257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaacgcttc agcaggcccg ccggccgagc cgcaccgcct
      121 gccccgcgtg accagcttcc gcacacgccg atcgacgctg tccggcggcc agcagggaac
      181 ctgggaccgc ctgtggccca agctcggcat gcaggccaga gacgaggatt cccagcccat
      241 aaccgacctg gacaccgagg cctggttcgg acgctcggca ccgttggtcc tggagatcgg
      301 ctcgggcacc gggacgtcca cgctcgagat ggccaaggcc gaaccgcaca tcgacgtgat
      361 cgccgtcgag gtgtaccgga agggcctcgc gcagttgctc agcgcgatcg accgcgaggg
      421 tgtcaccaac atccggatga ttcgcggcga cggcgtcgac gttctcgagc acatgatcgg
      481 cagcaacacc ctgaccggtg tgcgggtctt cttcccggac ccgtggccca aggcccgcca
      541 ccacaagcgc cggctgctgc aacctgccac ggtcgcgctc atcgccgacc ggttgcgcga
      601 gggcggcgtc ctgcacgccg cgaccgacca catgggatac gccgagcaca tcgccgaggt
      661 cggcgatgcc gagccgctgc tgcgccgggt gtcgaccagc gacgacctgc cgatatccct
      721 cgagcggccc gtcacgaaat accagcgcaa ggccctggcc ggcaccgagg tggccgaact
      781 ggtgtgggag aaacgcctaa agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
      841 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
      901 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
      961 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1021 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     1081 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     1141 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     1201 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     1261 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     1321 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     1381 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     1441 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     1501 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     1561 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     1621 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     1681 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     1741 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     1801 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     1861 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     1921 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     1981 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     2041 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     2101 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     2161 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     2221 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     2281 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     2341 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     2401 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     2461 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     2521 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     2581 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     2641 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     2701 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2881 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     3181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3361 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3421 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3481 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     3541 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     3601 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     3661 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     3721 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     3781 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     3841 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     3901 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     3961 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4021 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     4081 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     4141 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     4201 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     4261 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     4321 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     4381 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     4441 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     4501 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     4561 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     4621 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     4681 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     4741 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     4801 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     4861 cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc
     4921 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc
     4981 aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc
     5041 cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc
     5101 cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc
     5161 gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc
     5221 ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc
     5281 acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc
     5341 cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc
     5401 tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg ggaggcggca
     5461 cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca
     5521 tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg tctctgacga
     5581 gcgggagaac tccctatcag
//
