LOCUS       sequence-315588-        5948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0508:Dendra
FEATURES             Location/Qualifiers
     source          1..5948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1892..1915
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2172..2972
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3097..3685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3586..3605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggctgccat ccgattcgag aaggcccagc ttcggtaccc
      121 tcacgccccc acgccggcgg tggcagagct ggacctagac atcgccgacg gtgaattcat
      181 ggtgctggtc ggcccgtccg ggtgcggcaa gtcgacgacg ctgcgcatgc tggccggcct
      241 ggaggacctc accagcggct cgatctacct ggacgaccgc gacatcacca cgacaccgcc
      301 gcaggaccgc gacatcgcga tggtgttcca gaactacgcc ctgtacccgc acatgacagt
      361 ggcaggcaac atggagttct gcctcaagaa cgcgggcctg tccaaggacg aacgccgccg
      421 ccgggtggct gctgccgccg aaaccctcgg cctcaccgag tatctcaacc gcaagcccaa
      481 ggcgctgtcg ggtgggcagc ggcaacgtgt cgcgatgggc cgcgcgatcg tgcgaaaccc
      541 gcaggtgttc tgcatggacg aaccactgtc caacctcgac gccaagatgc gtgtgcagac
      601 ccgcaccgac atcgccaaac tgcaacgcga actcggcgtc accaccatct acgtcacgca
      661 tgaccagacc gaggccatga cgatggggga tcgtgtcggc gtcatgaaag acggtgttct
      721 gcaacaggtc ggcgcaccca tggacctcta cgaccggccg gccaacgcat tcgtcgcggg
      781 cttcatcggg tcgccggcca tgaagttctt cgacgcccag ctcggtgacc gcacggtcgg
      841 cgtggccggg cacgacatgg ccgtgcagtg cgctgccgac ttgcgtggcc ccgtgctgat
      901 cggcgtgcgc ccggaaggat ggcggctcac cgcacccgat gccggcatcg ccgtgaaggt
      961 cggtgtggtc gaggtactcg gcgccgattc gtatctgtac ggcaccgcga tccatgccgg
     1021 ggccgacgtc gacgtcgtcg tgcgcgtcag cacgcgcgac ggtatccgcg ccgacgacac
     1081 cgtgcacgtc accgccgacc cggacgcgat ccacctgttc gacaacgaaa cgggcgtgcg
     1141 gctcaataag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga
     1201 catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg
     1261 ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg
     1321 cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt
     1381 gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata
     1441 ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat
     1501 ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc
     1561 gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca
     1621 cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg
     1681 ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga
     1741 cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt
     1801 caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga
     1861 aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt
     1921 taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg
     1981 atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     2041 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag
     2101 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
     2161 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
     2221 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
     2281 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
     2341 tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
     2401 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
     2461 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
     2521 ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa
     2581 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
     2641 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     2701 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     2761 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     2821 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     2881 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg
     2941 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     3001 gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg
     3061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3241 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3481 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     3541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3781 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3841 cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg
     3901 cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg
     3961 ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt
     4021 ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag
     4081 gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac
     4141 cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg
     4201 ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag
     4261 ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac
     4321 cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag
     4381 cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag
     4441 ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca
     4501 tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg
     4561 gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct
     4621 ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg
     4681 aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg
     4741 gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg
     4801 gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac
     4861 aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg
     4921 tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag
     4981 ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg
     5041 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
     5101 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
     5161 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
     5221 gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc
     5281 ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag
     5341 cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc
     5401 gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac
     5461 acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc
     5521 gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag
     5581 gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc
     5641 gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag
     5701 cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt tgatcgggga
     5761 cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg atccgcgacg
     5821 tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc ccggcgttga
     5881 tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc gggagaactc
     5941 cctatcag
//
