LOCUS       sequence-315601-        6755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0028:Dendra
FEATURES             Location/Qualifiers
     source          1..6755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2699..2722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2979..3779
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3904..4492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4393..4412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaccacgcc tcagcacctt tccgaccgat acgaactcgg
      121 tgagatcctc ggcttcggcg gcatgtccga agtccacctg gcgcgcgatc tgcgccttca
      181 ccgcgatgtc gcggtgaaag tgctgcgcgc cgaccttgcg cgcgacccga gcttctacct
      241 gcggttccgc cgcgaggcac agaacgccgc ggcgctgaac caccccgcga tcgtcgcggt
      301 gtacgacacc ggcgaggccg agacacccaa cgggccgctg ccgtacatcg tcatggagta
      361 cgtcgacggt gtgaccctgc gcgacatcgt ccacaccgac ggtccgatcg cgccgcggcg
      421 tgcgatcgag atcatcgccg acgcgtgcca ggcgctgaac ttcagccacc agcacggcat
      481 catccaccgc gatgtcaagc cggccaacat catgatcagc aagaacaacg ctgtgaaggt
      541 gatggacttc ggcatcgccc gcgcgctggc cgacaccggc aacagcgtca cgcagaccgc
      601 cgcggtgatc ggcaccgcgc agtacctgtc acccgagcag gcgcgcggtg agaccgtcga
      661 tgcgcgctcg gacgtgtact cgctgggctg cgtgctctac gagatcctca ccggtgaacc
      721 gccgttcatc ggggattcgc ccgtcgcggt cgcctatcag cacgtgcggg aggatccggt
      781 gccgccgtcg cggcgccatg ccgacgtcac gcccgaactg gacgccgtgg tgctcaaggc
      841 gctggccaag aaccccgaca accggtacca gacggccgcc gagatgcgcg ccgacctcat
      901 ccgtgtgcac gagggccagg cgcccgacgc gcccaaggtg ctcaccgacg ccgagcgcac
      961 gtcgatgctg gccgccccgc ccgccgaccg cgcgggcgcg gccacccagg acatgcccgt
     1021 gccgcgtccg gccgggtaca gcaagcagcg cagcacctcg gtggcgcgct ggctcatcgc
     1081 ggtcgcggtg ctcgcggtgc tgaccgtggt ggtgacggtc gcgatcaaca tggtcggcgg
     1141 caacccgcgc aacgtccagg tgcccgacgt cgccgaacag tccgccgacg acgcgcaggc
     1201 cgcgctgcag aaccgcggct tcaagaccgt catcgaccgc cagcccgaca acgaggtgcc
     1261 gcccggcctg gtgatcggaa ccgatcccga ggcgggttcc gagctgggcg cgggggagca
     1321 ggtcaccatc aacgtctcga cggggcccga gcaggccctt gtgcccgacg tggcaggcct
     1381 gaccccgacg caggccaggc agaagctcaa ggacgccggg ttcgagaagt tcagggagtc
     1441 gccgagcccg tcgacgcccg agcagaaggg ccgggtgctc gcgaccaacc cgcaggccaa
     1501 ccagaccgcg gcgatcatca acgagatcac gatcgtcgtg ggtgcgggcc cggaggatgc
     1561 accggtgctc agttgcgccg ggcagaacgc cgagagctgc aaggcgatcc tggccgcggg
     1621 cggtttcacc aacaccgtcg tcgtcgaggt cgacaacccg gccgcggccg gtcaggtggt
     1681 cggtaccgaa ccggccgacg gccagtcggt gcccaaggac accgtgatcc agatacgggt
     1741 gtcgaagggc aaccagttcg tgatgcccga cctggtcggc cagttctggt cggacgccta
     1801 tccgcggctg accgcgctgg gctggaccgg tgtgctcgac aagggacccg acgtccgcga
     1861 cagcggtcag cgcaccaacg ccgtggtgac gcagagccct tccgcgggaa cgccggtgaa
     1921 caaggacgcc aagatcacgc tgagcttcgc tgcgaagctt ggtgcggccg cgggcaacac
     1981 gcctggcatc aatctgatca aggaggacat gcgtgtcaag gtgcatatgg agggcaacgt
     2041 gaacggtcac gccttcgtga tcgaggggga gggtaagggt aagccttacg aagggaccca
     2101 gaccgccaac ctcaccgtca aggagggcgc ccctctccca ttctcgtacg atatcctcac
     2161 taccgcggtg cattacggga accgcgtgtt caccaagtac cccgaggata tccctgacta
     2221 cttcaagcag agcttcccgg agggatactc ctgggagcgg acgatgacct tcgaagacaa
     2281 gggtatctgt acgatccgca gcgacatctc gctggaaggg gactgtttct tccagaacgt
     2341 ccgtttcaag gggaccaact ttccgccgaa cggcccggtc atgcagaaga agaccctcaa
     2401 gtgggagccg agcacggaga agctgcacgt tcgggacggc ctcctggtcg gcaacatcaa
     2461 catggcgctg ctgctggaag gcggcggcca ctacctatgc gacttcaaga ccacgtacaa
     2521 agcgaagaag gtcgtccagc tgcccgacgc gcacttcgtg gaccaccgca tcgaaatcct
     2581 ggggaacgat tcggactaca acaaggtcaa gttgtacgag cacgctgtgg cacgctatag
     2641 cccgctcccg tcgcaggtct ggttcgaagg cggcggcggg gagaacctgt atttccagga
     2701 ctacaaggac gacgacgaca agtgatttaa ataacccctt ggggcctcta aacgggtctt
     2761 gaggggtttt ttgttaacta gcgtacgatc gaattcctgc agctcacggt aactgatgcc
     2821 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
     2881 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
     2941 atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg gtggagtgca
     3001 atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc aaccttgggg
     3061 ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg cccctcgaag
     3121 atgggccact tggactgatc gaggccctgc gtgctacgct gggtccggga gggacgctcg
     3181 tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg tcgcccgtta
     3241 caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta aagcgcagcg
     3301 cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc tctgatccat
     3361 tgcccctgcc accttactcg cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc
     3421 aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt gccgagttga
     3481 tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat ggcaagttgg
     3541 tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg gcggacaggt
     3601 ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt gctcggttga
     3661 tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat ccgttgatct
     3721 tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
     3781 gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
     3841 caacacgggg gatccactag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc
     3901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4021 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4321 ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4621 tcgccgcagc cgaacgaccg agcgcaacgc gtgcggccgc ggtacccggg gatcctctag
     4681 agtcgaccac caagggcacc atctctgctt gggccacccc gttggccgca gccagctcgc
     4741 tgagagccgt gaacgacagg gcgaacgcca gcccgccgac ggcgagggtt ccgaccgctg
     4801 caactcccgg tgcaaccttg tcccggtcta ttctcttcac tgcaccagct ccaatctggt
     4861 gtgaatgccc ctcgtctgtt cgcgcaggcg gggggctcta ttcgtttgtc agcatcgaaa
     4921 gtagccagat cagggatgcg ttgcaaccgc gtatgcccag gtcagaagag tcgcacaaga
     4981 gttgcagacc cctggaaaga aaaatggcca gagggcgaaa acaccctctg accagcggag
     5041 cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga atgggtgtct gccgaccaca
     5101 tatgggccgg tcaagatagg tttttacccc ctctcggctg catcctctaa gtggaaagaa
     5161 attgcaggtc gtagaagcgc gttgaagcct gagagttgca caggagttgc aacccggtag
     5221 ccttgttcac gacgagagga gacctagttg gcacgtcgcg gatggggatc gctgaagact
     5281 cagcgcagcg ggaggatcca agcctcatac gtcaacccgc aggacggtgt gaggtactac
     5341 gcgctgcaga cctacgacaa caagatggac gccgaagcct ggctcgcggg cgagaagcgg
     5401 ctcatcgaga tggagacctg gacccctcca caggaccggg cgaagaaggc agccgccagc
     5461 gccatcacgc tggaggagta cacccggaag tggctcgtgg agcgcgacct cgcagacggc
     5521 accagggatc tgtacagcgg gcacgcggag cgccgcatct acccggtgct aggtgaagtg
     5581 gcggtcacag agatgacgcc agctctggtg cgtgcgtggt gggccgggat gggtaggaag
     5641 cacccgactg cccgccggca tgcctacaac gtcctccggg cggtgatgaa cacagcggtc
     5701 gaggacaagc tgatcgcaga gaacccgtgc cggatcgagc agaaggcagc cgatgagcgc
     5761 gacgtagagg cgctgacgcc tgaggagctg gacatcgtcg ccgctgagat cttcgagcac
     5821 taccggatcg cggcatacat cctggcgtgg acgagcctcc ggttcggaga gctgatcgag
     5881 cttcgccgca aggacatcgt ggacgacggc atgacgatga agctccgggt gcgccgtggc
     5941 gcttcccgcg tggggaacaa gatcgtcgtt ggcaacgcca agaccgtccg gtcgaagcgt
     6001 cctgtgacgg ttccgcctca cgtcgcggag atgatccgag cgcacatgaa ggaccgtacg
     6061 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
     6121 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
     6181 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
     6241 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
     6301 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
     6361 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
     6421 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
     6481 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
     6541 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
     6601 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
     6661 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
     6721 aagggtctct gacgagcggg agaactccct atcag
//
