LOCUS       sequence-315603-        5618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0244:Dendra
FEATURES             Location/Qualifiers
     source          1..5618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1562..1585
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1842..2642
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2767..3355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3256..3275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcgggtgga catgaccccc gccagcggtg agggatcgcg
      121 ggcgcccgag ggcgtcaatc gcacggtcct catggtcgac gacgacccgg atgtccgaac
      181 ctcggtggcg cgcggcctgc gccactcggg gttcgacgtc cgggtcgcgg cgtccggcaa
      241 ggaagcgctg cggctgctgt cgacggagtc gcacgatgcc ctcgtactgg acgtgcagat
      301 gccggagctc gacggcgtcg ccgtggtcac cgcgttgcgg gcgctgggca acgacatccc
      361 catctgtgtg ctgtcggcac gcgacaccgt caacgaccgg atcgccggtt tggaggcagg
      421 tgccgatgac tacctgacca aaccgttcga cctcggtgaa ctggtggccc ggctgcatgc
      481 gctgctgcgg cgcgcgagcc acaccgagca gccgtccgac accatgacag tcggaccggt
      541 caccatcgac accgcgcgca ggctggtgtt cgtcagcggt gaacgcgtcg acctgaccaa
      601 gcgcgagttc gacctgctgg cggtgctcgc cgagaacgcg ggcgtggtgc tgtcccggca
      661 gcggttgctg gagctggtgt ggggctacga cttcgacgtc gacaccaacg tggccgacgt
      721 cttcatctcc tatctgcggc gcaaactcga acgcgacggc gtgccgcgtg tgatccacac
      781 cgtgcgcggc atcgggtacg tgctgcggga agagccgaag cttggtgcgg ccgcgggcaa
      841 cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata tggagggcaa
      901 cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt acgaagggac
      961 ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt acgatatcct
     1021 cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg atatccctga
     1081 ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga ccttcgaaga
     1141 caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt tcttccagaa
     1201 cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga agaagaccct
     1261 caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg tcggcaacat
     1321 caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca agaccacgta
     1381 caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc gcatcgaaat
     1441 cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg tggcacgcta
     1501 tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc tgtatttcca
     1561 ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct ctaaacgggt
     1621 cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac ggtaactgat
     1681 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
     1741 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
     1801 actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt
     1861 gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg
     1921 gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg
     1981 aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg ggagggacgc
     2041 tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg
     2101 ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat gtaaagcgca
     2161 gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc atctctgatc
     2221 cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg
     2281 ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat cttgccgagt
     2341 tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag gatggcaagt
     2401 tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc ttggcggaca
     2461 ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc tttgctcggt
     2521 tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga gatccgttga
     2581 tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg
     2641 gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg
     2701 gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     2761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2881 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3181 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3481 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     3541 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     3601 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     3661 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     3721 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     3781 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     3841 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     3901 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     3961 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     4021 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     4081 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     4141 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     4201 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     4261 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     4321 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     4381 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     4441 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     4501 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     4561 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     4621 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     4681 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     4741 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     4801 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     4861 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     4921 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     4981 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     5041 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     5101 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     5161 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     5221 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     5281 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     5341 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacgg
     5401 tgaccacaac gcgcccgctt tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca
     5461 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
     5521 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
     5581 gagaagggtc tctgacgagc gggagaactc cctatcag
//
