LOCUS       sequence-315604-        5894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0339:Dendra
FEATURES             Location/Qualifiers
     source          1..5894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1838..1861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2118..2918
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3043..3631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3532..3551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcagctcgg atattgggct gcgcagccgc cgagcaacca
      121 tgcggagttg gtggccgcgg ccgaggatgc cggtttcgac accgtgttca ccgccgaggc
      181 gtggggctcg gatgcgttca cgccgctggc ctggtggggc cgtgagacca gccggctgcg
      241 gctgggcacg tcggtggtgc agatgtcggc gcgcactccc acggcgtgtg ccatggcgtc
      301 gctgacgctg gaccatctct cgggtggccg gcacatcctg gggctggggg tgtccgggcc
      361 gcaggtcgtg gagggctggt acggccagaa gtttccgaaa ccgctggcgc gtacccgcga
      421 gtacgtcgac atcatccggc aggtgtgggc ccgcgaggcc ccggtgacca gcgacggacc
      481 gcactatccg ctaccgctgg ccggtgcggg caccaccgga ctcggcaagg cgctcaagcc
      541 catcacgcat ccgctgcgcg cggacatccc gatcatgctg ggcgccgaag ggcccaggaa
      601 cgtcgcgctg gccgccgaga tctgcgacgg ctggctgccg atcttctaca cgccgcgcat
      661 ggccgacacc tacaacgcgt ggctcgacga aggttttgcc aagccgggtg cgcgtcgcac
      721 gcgcgagacc ttcgagatct gcgcgacggc caacatcgtc atcaccgagg accgggccgc
      781 ggcgttcgcg gcgatgaagc cctacctcgc gctgtacatg ggcggtatgg gtgccgagga
      841 caccaacttc cacgccgacg tctaccgccg catgggctac ggcgaggtcg tcgacgaggt
      901 cacaaggctg ctgcgcagca gaggtaaaga ggaagctgca caagcaattc ccgacgaggt
      961 cgtcgacgac gccgcgatcg tcggcgacgt ggcctacgtg cgtgaacaga tcaaggtgtg
     1021 ggaggccgcg ggcgtcacca ccatggtcgt caatgcccgc agtcccgaac acatccacca
     1081 attgacttca ctgaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1141 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1201 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1261 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1321 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     1381 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     1441 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     1501 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     1561 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     1621 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     1681 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     1741 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     1801 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     1861 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     1921 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     1981 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2041 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2101 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2161 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2221 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2281 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     2341 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     2401 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     2461 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     2521 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     2581 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     2641 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     2701 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     2761 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     2821 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     2881 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     2941 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3001 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3181 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3421 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     3481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3781 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     3841 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     3901 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     3961 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4021 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4081 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4141 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4201 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4261 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4321 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4381 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     4441 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     4501 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     4561 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     4621 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     4681 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     4741 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     4801 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     4861 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     4921 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     4981 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5041 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5101 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5161 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5221 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
     5281 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
     5341 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
     5401 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
     5461 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
     5521 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
     5581 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
     5641 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
     5701 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
     5761 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
     5821 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
     5881 gaactcccta tcag
//
