LOCUS       sequence-315605-        6146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0389:Dendra
FEATURES             Location/Qualifiers
     source          1..6146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2090..2113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2370..3170
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3295..3883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3784..3803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaaatttgt gctggcgagt tacgggacgc gaggcgacat
      121 cgaaccttcc gtcgttgtcg gtcgcgaact gcagagccgg ggccataccg tcctgatggc
      181 tgtgccgcct gacctgatcg agttcacgga ggccgcgaac ttgaggaccg tggcctacgg
      241 cctggacacg aaaacgtggc tggatatcta ccgcaacttc tggaccttcg ccctgcacag
      301 attctggaag gcccgggaaa ttcggtcgtt gtggcgtgag atgtgggacc tcagtgacga
      361 atgctgggca cagatgaacg ccactctcag gtcggtggct gccgatgcgg acgtcttgtt
      421 cgccggacag agttatcaag agcctgccgc gaatgtggcg gaattctacg atgttccact
      481 cgtcacgctg caccacatcc cgatgcgacc caacggccgg cttgtcacgt tcctgccgcc
      541 ggtactggcg cgcgcatcga tgaccgcgtt cgattggttc gcttggcggt tgaacaagaa
      601 agtcgaggac gcgcagcggc gcgacctcgg acttccgaaa gcgactggcc cgtcgacaca
      661 acggatcgcc gaccggatgt ctctggaagt tcaggcatac gacgaggtct gctttccagg
      721 gctggcggag gaatgggcga aatgggggga gctgcgtcca tacgtgggca ccctcacgat
      781 ggaactcacc accccggcgg atgaagaagt gtcgtcgtgg atatgcggcg gcacgcctcc
      841 gatctgcttc ggcttcggca gcatgccggt ggaatcgccg gctgacactg tggcgatgat
      901 cagctcagtt tgtgcagagc tgggcgagcg agcgctgatc tgtgccggct ggagcgactt
      961 cagcgacgtc gcgctcgccg atcacgtgaa ggtcgtcggc gcagtcaact acgccaccgt
     1021 gttcccgggt tgccgcgcag ttgtgcatca cgggggttcc gggaccaccg cggccagcct
     1081 ccgcgccggg ctgcccacgc tggtcctgtc gatggatgcc aaccaggctc tctggggtgc
     1141 ccagatcaag cgtttgaaag tcggtaccac gcggcggttc tcgaagacga atcggcggac
     1201 gctgatcgcc gacttgcgta cgattctcga tccggcgtat gcggctcgcg ctcaagctct
     1261 cgcacgagga atgaccagac cggccgagag tgtcgtcaga gcggccgatg tcatcgaggc
     1321 cttcgcgagg tcgaggtgtt cggcgaagct tggtgcggcc gcgggcaaca cgcctggcat
     1381 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
     1441 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
     1501 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
     1561 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
     1621 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
     1681 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     1741 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     1801 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     1861 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     1921 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     1981 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     2041 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     2101 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     2161 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     2221 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     2281 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     2341 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     2401 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     2461 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     2521 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     2581 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     2641 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     2701 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     2761 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     2821 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     2881 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     2941 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     3001 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     3061 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     3121 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     3181 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     3241 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3421 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     3721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4021 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     4081 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     4141 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     4201 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     4261 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     4321 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     4381 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     4441 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     4501 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     4561 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     4621 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     4681 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     4741 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     4801 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     4861 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     4921 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     4981 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     5041 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     5101 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     5161 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     5221 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     5281 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     5341 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     5401 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     5461 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     5521 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     5581 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     5641 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     5701 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     5761 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     5821 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     5881 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     5941 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     6001 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     6061 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     6121 tgacgagcgg gagaactccc tatcag
//
