LOCUS       sequence-315609-        5522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0739:Dendra
FEATURES             Location/Qualifiers
     source          1..5522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1466..1489
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1746..2546
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2671..3259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3160..3179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggctgaggc cgtcggcccc accgagtggg gagacagtcc
      121 cggcgtcggc ccgtgggagg ggccgtggcc cgacgacccg cgttacgacc ccgacctgct
      181 gcgtgacggt gactaccgca atgtggttga cgcctaccgg tattggaccc gcccggcgat
      241 cgtcgccgac atcgacacgc gcagacaccc gctgcacatc gcgatcgaga acttcggcag
      301 cgacgccaac atcggcacgg tggtgcgcac cgcgaacgcg ttcgcggtcg acaccgtcca
      361 catcgtgggg cggcggcgct ggaaccggcg cggtgcgatg gtgaccgacc gctaccagcg
      421 gctgcgccac cacgacacca ccgcggacct gctcgatttc gccgacgatg caggcctggt
      481 ggtcgtggcg gtggacaacg tccccggatc ggtgcggctc gaggaaaccg aactgcccag
      541 gaactgcctg ctcatcttcg gtcaggaagg tccgggcatc acgccggagg cccaggccgg
      601 tgccgcggtc acggtgtcga tcgcgcagtt cggctcgacc cgcagcatca acgccggtgt
      661 tgccgcgggc atcgccatgc acgcctggat cacccgacat gccgacatgt cgaaggcgtg
      721 gaagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
      781 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
      841 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
      901 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
      961 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
     1021 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     1081 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     1141 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     1201 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     1261 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     1321 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     1381 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     1441 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     1501 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     1561 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     1621 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     1681 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     1741 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     1801 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     1861 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     1921 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     1981 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     2041 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     2101 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     2161 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     2221 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     2281 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     2341 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     2401 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     2461 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     2521 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     2581 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     2641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3061 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     3121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     3421 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     3481 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     3541 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     3601 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     3661 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     3721 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     3781 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     3841 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     3901 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     3961 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     4021 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     4081 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     4141 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     4201 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     4261 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     4321 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     4381 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     4441 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     4501 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     4561 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     4621 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     4681 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     4741 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     4801 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     4861 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     4921 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     4981 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     5041 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     5101 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     5161 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     5221 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     5281 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     5341 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5401 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     5461 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     5521 ag
//
