LOCUS       sequence-315611-        5381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0048:Dendra
FEATURES             Location/Qualifiers
     source          1..5381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1325..1348
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1605..2405
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2530..3118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3019..3038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgggtaagaa cgagcgcacg aagatcgtga tgtccgacga
      121 ggagatcgcc gaattcgtcg agcggagtcg cacggcgacc atggccaccg tattgcccga
      181 cggcaggccg catctggtgg cgatgtggta cgcggtggtg gacggcgaga tctggttcga
      241 gaccaaggcc aagtcccaga aggccgtcaa cctgcgtcgc gatccgacgg tcaccgtcct
      301 catcgaggac ggccacacct acgacaccct gcggggcgtg tcgatcgacg gcaccgccga
      361 gatcgtggat gacccggaaa cgcttctgcg cgtgggcatc agcgtgtggg agcggtacac
      421 cggtccctac accgacgaga tgcggccctt cgtcgaccag atgatgaaca atcgcatcgc
      481 cgtgcgcgtc gtccccggcc gtacccgcag ctgggatcac cgtaagctgg gtatgcccgc
      541 gatgccgttg ggcggcagca ccgcgcagta cctgaactcg aagcttggtg cggccgcggg
      601 caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg
      661 caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg
      721 gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat
      781 cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc
      841 tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga
      901 agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca
      961 gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac
     1021 cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa
     1081 catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac
     1141 gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga
     1201 aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg
     1261 ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt
     1321 ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg
     1381 ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact
     1441 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
     1501 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
     1561 ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg
     1621 agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc
     1681 ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc
     1741 tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga
     1801 cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc
     1861 ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc
     1921 gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg
     1981 atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg
     2041 atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg
     2101 agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca
     2161 agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg
     2221 acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc
     2281 ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt
     2341 tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga
     2401 ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     2461 ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa
     2521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2641 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2941 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3181 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3241 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc
     3301 ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca
     3361 gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga
     3421 ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa
     3481 tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca
     3541 tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc
     3601 acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca
     3661 gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg
     3721 accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg
     3781 aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc
     3841 cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg
     3901 aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg
     3961 tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag
     4021 aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc
     4081 gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca
     4141 gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt
     4201 gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt
     4261 aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca
     4321 gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat
     4381 gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc
     4441 gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg
     4501 atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc
     4561 cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg
     4621 aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac
     4681 cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg
     4741 ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg
     4801 gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg
     4861 acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac
     4921 cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag
     4981 acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt
     5041 cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg
     5101 agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga
     5161 cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg
     5221 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
     5281 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
     5341 tgtgagaagg gtctctgacg agcgggagaa ctccctatca g
//
