LOCUS       sequence-315612-        6074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0317:Dendra
FEATURES             Location/Qualifiers
     source          1..6074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(239..250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2018..2041
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2298..3098
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3223..3811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3712..3731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaaccgcgc tgtggcgctg cgtatcgcgg catgtggact
      121 tctggggctc ggtgctgccc tgctgatcgc cgcgttgctg ttgaccacct acaccaaggg
      181 caagatcgcc aagatcccgc tcgacatcga cacgtcgttg gtcagtgacg ggacggcgac
      241 ggccttcgat ccggactcgc tggtcgccga acggttcaag atcgaccgtg acgtgccggt
      301 ggccctgcaa cagcagatga gcgtcgaagc gccgtccaac gccgacgtcg tgacgttcca
      361 ggtgggcacc acgctgcgcc gcaccgaccg tcagcaggac gccgggctgc tgctcgcgct
      421 ggtggacacc gtcacgatga accggaacac ggccgaggcc gtgtcgagcg agaacaaccc
      481 gggtggcgcc gtgcagaagc cgcgcgccat cgaggacgag aagccgccca ccaacatcgc
      541 gctgccgcac gagggcctga cctaccgctt cccgttcgac accgagaaga agacctatcc
      601 gttcttcgac ccgatcgcgc agaaggcgtt cgacgccaac tacgacggcg aggaagacgt
      661 caacgggctg accacctacc ggttcgtcca gaacgtcggc tacgacgccg acggcaagct
      721 cgccgacccg atcaagtact cctcgctgta cgaggacgac gcagacgcgt cggtcaccgc
      781 gcgcgccgag gtgtggggtg tgccgggcga accggacgag tccatcacga tggaccggtt
      841 ctacgccgct tcgcgcacgt tctgggtcga cccagtgtcg ggcacgatcg tcaagagcga
      901 agaacacggc taccagtact acgcacgtga ggccctcaag cccgaggtca cctacgtgga
      961 cttcaaggtc accaccaacg aggagagcgt cgaatcgcag gttgccgcag cgtccgacga
     1021 gcgtgaccgc atcgcgctgt ggacacggat cctgccgatc acgttcaccg cgctcggcct
     1081 ggtgtcgctg gtcggcggcg cggtcctcgg ttcgttcgcg ctgcgcaccg agtccacgct
     1141 catcgatccg ggtctcgaca ccgccgacca cggattcttc gatacgcagg gcatccaggt
     1201 gccgggtgcg gaggccaaga ccgagaagct gcccgcgcag cgtccgaccg atctgccacc
     1261 ggaccgaccg atcaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1321 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1381 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     1441 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     1501 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     1561 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     1621 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     1681 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     1741 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     1801 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     1861 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     1921 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     1981 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     2041 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     2101 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     2161 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2221 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2281 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2341 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     2401 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     2461 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     2521 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     2581 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     2641 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     2701 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     2761 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     2821 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     2881 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     2941 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3001 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3061 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     3121 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3181 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3361 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3601 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3961 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     4021 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     4081 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     4141 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4201 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4261 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4321 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4381 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     4441 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     4501 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     4561 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     4621 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     4681 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     4741 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     4801 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     4861 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     4921 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     4981 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     5041 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     5101 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     5161 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5221 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5281 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5341 gtcgcggaga tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
     5401 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
     5461 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
     5521 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
     5581 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
     5641 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
     5701 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
     5761 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
     5821 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
     5881 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
     5941 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
     6001 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
     6061 gaactcccta tcag
//
