LOCUS       sequence-315616-        6647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0615:Dendra
FEATURES             Location/Qualifiers
     source          1..6647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2591..2614
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2871..3671
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3796..4384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4285..4304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggtcagccg tttcgccacc tgctgccgtg cgctgggcct
      121 gacggtcaac gaccggcagc gacccgccga cctgacggcg gcccgtgcgg gcttcgccgg
      181 tctgacccat ctcgcacacg accagtgcga cgcgtggatc gggctggccg cagccggtga
      241 ggtcaccccg gccgtcgtcg acgcggtgtg gcgcaccgtg gcctccgcgg gcgtgctgca
      301 gcgcgagatc ggtctcgccg ccggcgaact gggcttcacc tacgacacgg ggtggtacct
      361 gcagttccgc gccaccgaac cggatgactt ccaacttgcc tatgcggcaa ggctttacga
      421 ggcaggcgag ttcggcgagg ccgacggact ggtcggtgag atcctggcgc gccgccccgg
      481 ctggttcgac gcgaggtggc tgcaggtggc gatcaaccac cgcgcccaac gctggtcgga
      541 cgtcgtgcgc ctgctcaccc ccgtggtgac cctcccgtcc ttggacgacg tgacgtcgca
      601 cgccgtgcgc accgcgctgg gaatctcgct ggcccggctc gggatgttcg cgcccgccat
      661 gtcgtatctg gaggatcctg ccgggccgat cgaggtggcc gcggtcgacg gtgcgctggc
      721 caaggcgctc acgctgcgcg cgcagggtga ggacgacgag gccaccgagg tgctgcagga
      781 cctcttcgcc acccacccgg agaacacgca ggtcgaacag gccctgctgg acacgtcgtt
      841 cggcctcgtc acgaccacgt cggcacgtat cgaggcccgc tccgacccgt gggatcccga
      901 aaccgagccc agcgaggccg aattcgtcga tccgggcgcc aaggaccgca aggcgcacct
      961 gctgctcgag gccgaggccg agctcgccga attcatcggc ctcgaggagg tgaagttcca
     1021 ggtcgcccgg ctcaagagct cggtggccat ggccatccgg cgtcaggaac gcgggctggc
     1081 cgtcgcgcag cgcaccaacc acctggtgtt cgcgggaccg cccggcaccg gcaagaccac
     1141 gatcgcgcgt gtcgtcgcca agatctactg cgggctgggg cttctcaaga aggagaccgt
     1201 gcgcgaggtg caccgcgccg acctgatcgg ccagcacatc ggcgagaccg aagccaagac
     1261 caacgcgatc atcgacagtg cgctcgacgg cgtgctgttc ctcgacgagg catacgccct
     1321 ggtgtccacg ggcgccaaga acgacttcgg cctcgtcgcc atcgacaccc tgctggcacg
     1381 catggagaac gaccgcgacc ggctcgtcgt gatcgtcgcc gggtaccgca aggacctcga
     1441 cgcattcctc gacaccaacg aaggtctgcg ttcgcgcttc acgcgcagca tcgacttccc
     1501 gtcctacacc gcacccgaac tcgtcgagat cgcggtgcgc atggccgaga aacgcgacag
     1561 cgtgttcgag aaggccgccc acgacgacat ggagcgtctg ttcacgcatc tcgcgcaggc
     1621 caccaccccc gacgccaacg gtgtcgagcg ccgcagtctc gacatcgcgg gtaacgcgcg
     1681 cttcgtccgc aacctggtcg agcgctccga ggaggagcgc gaataccggc tcgaccactc
     1741 cgaccaagaa gatttcaccg acgaggagat gatgacgatc accgccggtg acgttcagcg
     1801 atcggccgcc ccgctgctgc gtggcctcgg actgtcggtg ccggcaaagc ttggtgcggc
     1861 cgcgggcaac acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat
     1921 ggagggcaac gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta
     1981 cgaagggacc cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta
     2041 cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga
     2101 tatccctgac tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac
     2161 cttcgaagac aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt
     2221 cttccagaac gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa
     2281 gaagaccctc aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt
     2341 cggcaacatc aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa
     2401 gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg
     2461 catcgaaatc ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt
     2521 ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct
     2581 gtatttccag gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc
     2641 taaacgggtc ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg
     2701 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     2761 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     2821 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
     2881 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
     2941 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     3001 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg
     3061 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     3121 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     3181 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     3241 tctctgatcc attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg
     3301 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     3361 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     3421 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     3481 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     3541 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     3601 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     3661 agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc
     3721 tgattgctgg ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4201 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4501 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg
     4561 gggatcctct agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg
     4621 cagccagctc gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg
     4681 ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag
     4741 ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg
     4801 tcagcatcga aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag
     4861 agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc
     4921 tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt
     4981 ctgccgacca catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
     5041 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
     5101 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
     5161 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
     5221 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
     5281 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
     5341 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
     5401 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
     5461 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
     5521 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
     5581 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
     5641 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
     5701 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
     5761 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
     5821 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
     5881 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     5941 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     6001 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt
     6061 cggccggaac tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca
     6121 ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg
     6181 aagtaccaga tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg
     6241 gccaagacct cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg
     6301 gggtttcttg tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct
     6361 gtgcggagac ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagaaa
     6421 tctagacggt gaccacaacg cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg
     6481 gcaccgtcac ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca
     6541 cggtgctgcg cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag
     6601 gcccggtgtg agaagggtct ctgacgagcg ggagaactcc ctatcag
//
