LOCUS       sequence-315617-        6071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0001:Dendra
FEATURES             Location/Qualifiers
     source          1..6071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2015..2038
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2295..3095
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3220..3808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3709..3728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcgacgac gacggctggg ctgaccgact tgaagtttcg
      121 cgtcgtccgc gaggacttcg cggatgcggt ggcctgggta gcccgcagct tgccgacccg
      181 gcccaccatc ccggtgttgg caggcgtcct gctgaccggc accgatgagg gcctgacgat
      241 ctcggggttc gactacgagg tctcggccga ggtcaaggtg agcgctgaaa tcgcttccgc
      301 cggaagcgtt ctggtgtccg gacggctgct gtccgacatc accaaggcgc tgcccgccaa
      361 gcctgtcgag gtcagcgtcg agggcacccg cgtgtccctg acctgcggca gcgcgcgctt
      421 ctcgctgccg acgctcgcgg tcgaggacta cccggcgctg cctgcgctgc ccgaggagac
      481 cggtgtgatc gcgtcggatc tgttcgccga ggccatcggc caggtggcgg tggcggcagg
      541 ccgcgacgac acgctgccga tgctgaccgg tatccgcgtg gagatctccg gcgagtccgt
      601 ggtgctcgcc gcgaccgacc ggttccgtct cgcggtgcgt gagctcacgt gggtcaccac
      661 ggcaggtgac gtcgaggccg cggtgctggt gccggcgaag acgctggccg aggccgccaa
      721 ggccggcacg gacggcaacc aggtgcatct ggcgctgggg tccggtgcgt cggtcggcaa
      781 ggacggtctg ttgggtatcc gcagcgaggg caagcgcagc acgacgcgcc tgctcgacgc
      841 cgagttcccg aagttccgcc agctgctgcc cgccgagcac accgcggtcg ccacgatcgg
      901 tgttgccgaa ctcaccgagg cgatcaagcg tgtggccctc gtggccgatc gcggcgccca
      961 gatccgcatg gagttcagcg acgacacgct caagctgtcc gcgggcgccg acgacgtggg
     1021 tcgtgccgag gaagacctcc cggtggattt cgcgggcgag ccgctcacca tcgcgttcaa
     1081 cccgacgtat ctcaccgacg gcctggggtc gctgcactcc gagcgcgtga ccttcgggtt
     1141 caccacaccg agccgtcctg cggtgttgcg gccggcaggc gaggatgatg gagccaacgg
     1201 tggttcgggt ccgttcccgg ccgccaagac ggattacgtg tatctgctga tgccggtgcg
     1261 gcttccgggc aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga
     1321 ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga
     1381 gggggagggt aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga
     1441 gggcgcccct ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg
     1501 cgtgttcacc aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg
     1561 atactcctgg gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga
     1621 catctcgctg gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc
     1681 gccgaacggc ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct
     1741 gcacgttcgg gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg
     1801 cggccactac ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc
     1861 cgacgcgcac ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa
     1921 ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt
     1981 cgaaggcggc ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg
     2041 atttaaataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt
     2101 acgatcgaat tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     2161 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     2221 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     2281 gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa
     2341 aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg
     2401 tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg
     2461 ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg
     2521 acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg
     2581 acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag
     2641 cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg
     2701 caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac
     2761 acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc
     2821 cgagacactg caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg
     2881 accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga
     2941 aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga
     3001 cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat
     3061 gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat
     3121 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc
     3181 atgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3361 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3601 acagcgtgag ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc
     3661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3901 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3961 caacgcgtgc ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct
     4021 ctgcttgggc caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga
     4081 acgccagccc gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc
     4141 ggtctattct cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg
     4201 caggcggggg gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc
     4261 aaccgcgtat gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa
     4321 tggccagagg gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg
     4381 tagctagttt ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt
     4441 taccccctct cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg
     4501 aagcctgaga gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc
     4561 tagttggcac gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc
     4621 tcatacgtca acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag
     4681 atggacgccg aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc
     4741 cctccacagg accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc
     4801 cggaagtggc tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac
     4861 gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct
     4921 ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc
     4981 tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac
     5041 ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag
     5101 gagctggaca tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg
     5161 gcgtggacga gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac
     5221 gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc
     5281 gtcgttggca acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc
     5341 gcggagatga tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca
     5401 ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg
     5461 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
     5521 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
     5581 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
     5641 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
     5701 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
     5761 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
     5821 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
     5881 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
     5941 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
     6001 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
     6061 ctccctatca g
//
