LOCUS       sequence-315618-        5960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0152:Dendra
FEATURES             Location/Qualifiers
     source          1..5960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1904..1927
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2184..2984
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3109..3697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3598..3617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacgttgat cggagtgccg cgggagtccg cggagggcga
      121 gcgccgcgtc gcgcttgtcc caaaagtcgt cgagaaattg agtgcccgag ggcttgaggt
      181 cgtcgtggag agtgcggccg gagcgggcgc gctgttcagc gacgcggatt acgagcgagc
      241 gggggccacg atcggtgatc cgtggccggc agatgtggtg gtgaaggtga atccacccac
      301 gtcggacgag atcagccagc tcaagcccgg ttcggtactc atcggcttcc tggccccgcg
      361 gacacagccc gagctcgcgt cgcggctgcg catcgcagat gtcacagcct ttgcgatgga
      421 atcgattccg cgcatatccc gtgcgcagac catggacgcg ttgtcgtctc aggccaacgt
      481 cgccgggtac aaggccgtgc tgttgggagc gtcgctgtcg acacggttcg tcccgatgct
      541 gaccaccgcg gcgggaacgg tcaaacccgc cagtgcgctg gtactcggag tcggcgtcgc
      601 gggcctgcag gcgctggcga ccgctaaacg cttgggcgcc aagacaaccg gatacgacgt
      661 gcgccccgag gtcgccgaac aggtccgttc ggtcggcgcg cagtggctcg atctggggat
      721 cgacgccgca ggcgagggcg ggtacgcgcg tgaactcagc gaggccgaac gtgcgcaaca
      781 gcagcaggcg ctcgaggacg cgatcaccaa gttcgacatc gtgatcacga ccgcgctggt
      841 gcccggccgt cccgcaccgc gtctggtgac cgccgccgcg gccaccggta tgcagccggg
      901 aagcgtcgtc gtcgatctcg ccggcgagac cggcggcaac tgtgagctca ccgagccggg
      961 acgcaccatc gtccaccacg gcgtcacgat cacctcgccg ctgaacctgc ccgcgaccat
     1021 gcccgagcac gccagcgagt tgtacgccaa gaacgtgacc gccctgctcg atctgctgct
     1081 caccgacgac ggcgtcgccc ccgatttcac cgacgagatc gtcgctgcct cctgcatcac
     1141 ccgcaccgaa ggggacatca agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
     1201 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     1261 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     1321 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1381 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     1441 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     1501 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     1561 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     1621 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     1681 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     1741 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     1801 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     1861 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     1921 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     1981 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     2041 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     2101 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     2161 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     2221 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     2281 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     2341 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     2401 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     2461 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     2521 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     2581 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     2641 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     2701 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     2761 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     2821 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     2881 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     2941 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     3001 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     3061 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3241 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     3541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3841 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     3901 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     3961 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     4021 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     4081 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     4141 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     4201 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     4261 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     4321 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4381 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     4441 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     4501 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     4561 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     4621 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     4681 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     4741 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     4801 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     4861 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     4921 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     4981 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     5041 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     5101 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     5161 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     5221 cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc
     5281 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc
     5341 aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc
     5401 cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc
     5461 cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc
     5521 gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc
     5581 ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc
     5641 acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc
     5701 cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc
     5761 tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg ggaggcggca
     5821 cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca
     5881 tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg tctctgacga
     5941 gcgggagaac tccctatcag
//
