LOCUS       sequence-31562-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-OMPDC
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     270..289
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     373..392
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        373..391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    392..416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             473..490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             500..517
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      121 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      181 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      241 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      301 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
      361 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      421 tagaaataat tttgtttaac tttaagaang gagatatacc atgggcagca gccatcatca
      481 tcatcatcac agcagcggcc tggttccgcg tggtagtatg cattttaaaa ctaaattaaa
      541 aaatcgaaga aagtgaagta aatacctgtt tgtgtattgg gttggatccc gatgaagatg
      601 atataaaaaa ctttatgaaa aatgaagaac aaaatggata taaaaatatc aaaaataaca
      661 tgaacagtaa taataatgga atagaaaata tcatcaaaat aggaaaggaa atactattaa
      721 cagatggaga aaatatacag aatttaagtg aagaagataa gtttttttat ttctttaacc
      781 acttttgttt ttatataatt aataacacaa aagaatatgc tttggtatat aaaatga
//
