LOCUS       sequence-315620-        5552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0029:Dendra
FEATURES             Location/Qualifiers
     source          1..5552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1496..1519
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1776..2576
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2701..3289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3190..3209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcaggtttt ggtggtcgac aactatgaca gcttcgtgtt
      121 caacctggtc cagtacctgg gccagctcgg tgtgcacgcg caggtctggc gcaacgacga
      181 cgcccggttg gccaccgagg acgcgatagc gcaggccgcc gccgacttcg acggtgtgct
      241 gctcagcccc gggccgggca cccccgaacg tgcgggagcc tccatcgcgc tggtgaaggc
      301 gtgcgccgcg gcgggcactc ccctgctggg cgtgtgcctg gggcatcagg cgatcggcgt
      361 ggcgttcggc ggcaccgtcg accgtgcgcc cgaactgctg cacggcaaga ccagcgtcgt
      421 gcaccacaac gacagcggcg tgctcaaggg tctgccggat ccgttcaccg cgacgcggta
      481 ccactcgctg acgatcctgc ccgagacgct gcccgccgcg ctcgaggtcg tcggccagac
      541 cgacaacggc atcatcatgg ccgtccggca caccgagctg ccgatccatg gcgtgcagtt
      601 ccaccccgag tcgatcctca cccagggcgg acaccgcatg ctggccaact ggctcggctt
      661 ctgcggcgcc gcaccggacg agacgctcgt gcgccgcctc gaggacgagg tcgcccgcgc
      721 ggtcgcggcc gctacgcagc gaagctcagc gaagcttggt gcggccgcgg gcaacacgcc
      781 tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg catatggagg gcaacgtgaa
      841 cggtcacgcc ttcgtgatcg agggggaggg taagggtaag ccttacgaag ggacccagac
      901 cgccaacctc accgtcaagg agggcgcccc tctcccattc tcgtacgata tcctcactac
      961 cgcggtgcat tacgggaacc gcgtgttcac caagtacccc gaggatatcc ctgactactt
     1021 caagcagagc ttcccggagg gatactcctg ggagcggacg atgaccttcg aagacaaggg
     1081 tatctgtacg atccgcagcg acatctcgct ggaaggggac tgtttcttcc agaacgtccg
     1141 tttcaagggg accaactttc cgccgaacgg cccggtcatg cagaagaaga ccctcaagtg
     1201 ggagccgagc acggagaagc tgcacgttcg ggacggcctc ctggtcggca acatcaacat
     1261 ggcgctgctg ctggaaggcg gcggccacta cctatgcgac ttcaagacca cgtacaaagc
     1321 gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac caccgcatcg aaatcctggg
     1381 gaacgattcg gactacaaca aggtcaagtt gtacgagcac gctgtggcac gctatagccc
     1441 gctcccgtcg caggtctggt tcgaaggcgg cggcggggag aacctgtatt tccaggacta
     1501 caaggacgac gacgacaagt gatttaaata accccttggg gcctctaaac gggtcttgag
     1561 gggttttttg ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac tgatgccgta
     1621 tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa
     1681 tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt
     1741 tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg
     1801 tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta
     1861 cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg
     1921 ggccacttgg actgatcgag gccctgcgtg ctacgctggg tccgggaggg acgctcgtca
     1981 tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac
     2041 cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc
     2101 atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc
     2161 ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg
     2221 tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg
     2281 caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac
     2341 gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc
     2401 tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc
     2461 gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc
     2521 tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag
     2581 ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt gctggggcaa
     2641 cacgggggat ccactagttc catgagcgtc agaccccgta gaaaagatca aaggatcttc
     2701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2821 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaggg
     3121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3361 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3421 ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt acccggggat cctctagagt
     3481 cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc agctcgctga
     3541 gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa
     3601 ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg
     3661 aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta
     3721 gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt
     3781 gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg
     3841 gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat
     3901 gggccggtca agataggttt ttaccccctc tcggctgcat cctctaagtg gaaagaaatt
     3961 gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag gagttgcaac ccggtagcct
     4021 tgttcacgac gagaggagac ctagttggca cgtcgcggat ggggatcgct gaagactcag
     4081 cgcagcggga ggatccaagc ctcatacgtc aacccgcagg acggtgtgag gtactacgcg
     4141 ctgcagacct acgacaacaa gatggacgcc gaagcctggc tcgcgggcga gaagcggctc
     4201 atcgagatgg agacctggac ccctccacag gaccgggcga agaaggcagc cgccagcgcc
     4261 atcacgctgg aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc agacggcacc
     4321 agggatctgt acagcgggca cgcggagcgc cgcatctacc cggtgctagg tgaagtggcg
     4381 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
     4441 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
     4501 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
     4561 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
     4621 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
     4681 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
     4741 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
     4801 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
     4861 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
     4921 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
     4981 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
     5041 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
     5101 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
     5161 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
     5221 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
     5281 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
     5341 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     5401 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     5461 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     5521 ggtctctgac gagcgggaga actccctatc ag
//
