LOCUS       sequence-315622-        6392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0392:Dendra
FEATURES             Location/Qualifiers
     source          1..6392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2336..2359
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2616..3416
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3541..4129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4030..4049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgttcagcct ccacgcgacg cggtctcggg cctccaccaa
      121 cgcgtttccg agggccatct tcgaagtatc tcgcacaact cgagaacgta cgactcgaac
      181 cgaaaagttc agtgattgcg atgcaagaca gcatctatgc cccgctcaag agcccgtggc
      241 tgcccgtgag gctattccgc cgaatctgac ccgatacggt ccgctcaggg ccgggtgggc
      301 gataattcct gcacacgcca acccgagagg tttgatccga atgaagtttg cgctggcatg
      361 ttacgggact cgcggcgacg tcgagccctc cgtcgcagtc ggccgcgagc tacagcgaag
      421 aggtcacgag gtttgcgttg cagtgccccc gggtctcgtc gctttcgccg aaacggccgg
      481 actagatgca gtggcgtatg ggcccgagtt ggacaatttt ctgcgcgacg atttccttcg
      541 caatttctgg atgcagatga tccgcaaccc tgttggctca cttcgcgaac tctgggcgcc
      601 cataacccgc tactggaacg atacgagcaa ggtgctcaca acgctcgcag acggtgccga
      661 tctgctgtcg acgggaataa atttcgagca ggcagccgcg aacgttgccg agcactacga
      721 cattccgttc gtctctctgc atcacttccc catgcgtccc aacggaaagc tggttccggc
      781 tctgccggcc tcggtggtcc gctccgtcgg cgccctctcc gagtggttgt tctggcgctc
      841 gacgaaagag gtcgaggatg cgcaacggcg cgaactcgga ttgccacgga cgaaggttcc
      901 gtccccgcgg aggatcgccg aacgcgggtc actcgagatt caggcctatg acgcggtgtc
      961 cgtacctggg ctggccgacg aatgggcaga tcaaaaccgc cagcggccgt tcacgggcgc
     1021 gctcacgatg agtttgtcga ccgaagccga cgacgacgtc acatcgtgga tcacatcggg
     1081 cgaaccgccg atctgctttg cgacaggcag tatccctgtc cagtcaccca cggccacgat
     1141 cgagatgatc agtcacgcgt gtgaccagct gggccagcga gcactggtct gtgccggcgg
     1201 caccgatctt cgcggtgttc cgcaatttga caacgtcaaa atggtgggcg cggtgaacta
     1261 tgcggcagtg ttcccattgt gccgcggaat cgtccatcac ggcggctcgg gtacgacggc
     1321 agcaagcctt cgcgccgggg tacccacctt gatcctctgg agcacagccg accaaccgta
     1381 ttggggcaac cagctcaaac gactgaaggt tgggacctcg cgccgctttt cgggcgcaag
     1441 tcgcaaatca cttgtgacgg acctgcgccg gattctccga cccgagtatg cggttcgggc
     1501 gcgcgagctc gccatgcgga tgaccccggc cgccgagagc gtgaccatcg ccgccgatct
     1561 ctatgaaaac gcggtgcgcc aagcggcgat gaagcttggt gcggccgcgg gcaacacgcc
     1621 tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg catatggagg gcaacgtgaa
     1681 cggtcacgcc ttcgtgatcg agggggaggg taagggtaag ccttacgaag ggacccagac
     1741 cgccaacctc accgtcaagg agggcgcccc tctcccattc tcgtacgata tcctcactac
     1801 cgcggtgcat tacgggaacc gcgtgttcac caagtacccc gaggatatcc ctgactactt
     1861 caagcagagc ttcccggagg gatactcctg ggagcggacg atgaccttcg aagacaaggg
     1921 tatctgtacg atccgcagcg acatctcgct ggaaggggac tgtttcttcc agaacgtccg
     1981 tttcaagggg accaactttc cgccgaacgg cccggtcatg cagaagaaga ccctcaagtg
     2041 ggagccgagc acggagaagc tgcacgttcg ggacggcctc ctggtcggca acatcaacat
     2101 ggcgctgctg ctggaaggcg gcggccacta cctatgcgac ttcaagacca cgtacaaagc
     2161 gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac caccgcatcg aaatcctggg
     2221 gaacgattcg gactacaaca aggtcaagtt gtacgagcac gctgtggcac gctatagccc
     2281 gctcccgtcg caggtctggt tcgaaggcgg cggcggggag aacctgtatt tccaggacta
     2341 caaggacgac gacgacaagt gatttaaata accccttggg gcctctaaac gggtcttgag
     2401 gggttttttg ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac tgatgccgta
     2461 tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa
     2521 tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt
     2581 tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg
     2641 tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta
     2701 cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg
     2761 ggccacttgg actgatcgag gccctgcgtg ctacgctggg tccgggaggg acgctcgtca
     2821 tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac
     2881 cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc
     2941 atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc
     3001 ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg
     3061 tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg
     3121 caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac
     3181 gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc
     3241 tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc
     3301 gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc
     3361 tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag
     3421 ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt gctggggcaa
     3481 cacgggggat ccactagttc catgagcgtc agaccccgta gaaaagatca aaggatcttc
     3541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3661 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaggg
     3961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4261 ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt acccggggat cctctagagt
     4321 cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc agctcgctga
     4381 gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa
     4441 ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg
     4501 aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta
     4561 gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt
     4621 gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg
     4681 gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat
     4741 gggccggtca agataggttt ttaccccctc tcggctgcat cctctaagtg gaaagaaatt
     4801 gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag gagttgcaac ccggtagcct
     4861 tgttcacgac gagaggagac ctagttggca cgtcgcggat ggggatcgct gaagactcag
     4921 cgcagcggga ggatccaagc ctcatacgtc aacccgcagg acggtgtgag gtactacgcg
     4981 ctgcagacct acgacaacaa gatggacgcc gaagcctggc tcgcgggcga gaagcggctc
     5041 atcgagatgg agacctggac ccctccacag gaccgggcga agaaggcagc cgccagcgcc
     5101 atcacgctgg aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc agacggcacc
     5161 agggatctgt acagcgggca cgcggagcgc cgcatctacc cggtgctagg tgaagtggcg
     5221 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
     5281 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
     5341 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
     5401 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
     5461 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
     5521 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
     5581 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
     5641 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
     5701 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
     5761 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
     5821 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
     5881 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
     5941 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
     6001 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
     6061 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
     6121 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
     6181 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     6241 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     6301 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     6361 ggtctctgac gagcgggaga actccctatc ag
//
