LOCUS       sequence-31563-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-HSP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     268..287
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    394..418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             433..455
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             466..483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             508..528
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
       61 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
      121 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
      181 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
      241 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
      301 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatcgatct
      361 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      421 tctagaaata attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
      481 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gcaattacca
      541 atatggaaac aagatgaaaa atcattaaca gaaaatgatt attatagttt ttataaaaat
      601 acatttaaag catatgatga tccattagct tatgttcatt ttaatgtaga aggacaaatt
      661 tcttttaatt ccattttata tattccaggt tctttaccat gggaattaag taaaaatatg
      721 tttgacgaag aatctagagg aataagatta tatgtaaaaa gagtttttat aaatgacaaa
      781 ttttctgaat ctataccacg ttggttaacc tttctaagag gtattgtaga tagtgaaaac
      841 ttaccattaa atgtagg
//
