LOCUS       sequence-31564-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-1Cys-PRX
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..186
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggttccgcgt
      181 ggtagtatgg gatatcattt aggagcaaca tttccaaatt ttacagccaa agcttcaggt
      241 atagatggtg attttgaatt atataaatat atagaaaata gttgggcaat attatttagt
      301 catccaaatg attttactcc agtatgtaca actgagttag cagaattagg aaaaatgcat
      361 gaagactttt taaaacttaa ttgtaaatta ataggcttta gttgtaattc aaaagagtca
      421 catgataaat ggatagaaga tataaaatat tatggaaaat taaataaatg ggaaattcct
      481 attgtttgtg atgaatcacg agaacttgca aataaattaa aaattatgga tgaacaagaa
      541 aaagatataa ctggtttacc attaacatgt agatgccttt tttttatttc tcctgaaaaa
      601 aaaattaaag caactgtatt atatccagca acaacaggta gaaatgctca tgaaatttta
      661 agagttttaa aatctttaca acttacttat accaccccag tagctacacc tgttaattgg
      721 aatgaaggtg acaaatgttg tgtcattcca actcttca
//
