LOCUS       sequence-315643-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control tet-inducible shRNA targeting eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Cotter KA, Gallon J, Uebersax N, Rubin P, Meyer KD, Piscuoglio S, 
            Jaffrey SR, Rubin MA
  TITLE     Mapping of m6A and Its Regulatory Targets in Prostate Cancer Reveals
            a METTL3-low Induction of Therapy Resistance.
  JOURNAL   Mol Cancer Res. 2021 Jun 4. pii: 1541-7786.MCR-21-0014. doi: 
            10.1158/1541-7786.MCR-21-0014.
  PUBMED    34088870
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2021 Jun 4. pii: 1541-7786.MCR-21-0014. doi: 
            10.1158/1541-7786.MCR-21-0014."
COMMENT     Sequence Label: GFP shRNA tet pLKO puro
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        127..221
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     138..157
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    175..193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    322..439
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        488..998
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     917..935
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
       61 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg
      121 gccgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatcccta
      181 tcagtgatag agacttataa gttccctatc agtgatagag acaccggtac aacagccaca
      241 acgtctatct cgagatagac gttgtggctg ttgtattttt gaattctcga cctcgagaca
      301 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      361 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      421 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg
      481 gctcgagggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
      541 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
      601 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
      661 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
      721 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
      781 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
      841 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
      901 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
      961 gctccctcgt tgaccgaatc accgacctct ctccccag
//
