LOCUS       sequence-315653-        7769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0576:Dendra
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3713..3736
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3993..4793
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4918..5506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5407..5426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgattcaagg cctgcacaac cgcagcgatt ccagaccgct
      121 tcttccccat gccctgaggg tcctggcaat cccggtgatc ctgttctggg tggcgttcgc
      181 cgccgtggtg aacatcatcg cgccgcagct ggagatcgtc ggcgaggagc attcggcacc
      241 gatggcgccc gaggatgcgc cgtcgctgac cggtatgcac cggatgggcg gcaacttcca
      301 ggagttcgac tccaacagca ccgtgatggt cgtcatcgag ggtcaggaac cgctcggccc
      361 cgacgcgcac gcgtactacg acgagatcat ccgcaaactc cggcaggatc cggaacacat
      421 tcagcacatc caggacttct ggggcgacac cctgacggcg gccggcgccc agagcgcgga
      481 cggcaaagcc gcatacgtga tgctgaacct cgcaggcgag caggggcaga cgctggccaa
      541 cgacggcgtg caagaagtgc ggcaggtgat cgccgacacc ccggcgccgc cgggcgtgca
      601 ggcctacgcc gcggggcccg cagccctcac cgacgacctg cacgtgatcg gcaacgccag
      661 cctcggcaag atcacgctgt tcacgctcgt cgcgatagcg gtcatgttgc tcatcgtcta
      721 ccgatcgatc gtcacgacgc tgatccagct cgtgctcacc ggtgtgggcc tgctggcctc
      781 gcgtggcctg atctcggtgc tcgcgacgca cgacgtgttc gggttgacca cgttcgccgg
      841 gaacatcctc acgatgctcg cgatcgcggc ggccaccgat tacggcatct tcctgttcgg
      901 ccggtaccgc gaggcccgcg gcgcgggcga ggaccccgag accgcctatt acacgacgtt
      961 caaatcggtg tcgccggtga tcgtggggtc gggtctgacg atcgcggggg cgacgtactg
     1021 cctgagtttc gcgcggctgc cctacttcag caccatgggc gccccggtcg cgatcggcat
     1081 gatcgtggtc gtcgccagtt cggtgaccat gggcccggcg gtgctgttcc tcggcagcaa
     1141 ggtcggcctg tacgaggcga aacgcgctgc caaaggccgc ttctggcgac gtgtcggcac
     1201 ggccgtcgtg cgctggcccg ccccgattct cgtcgcgagc gcgttcatcg tgctcatcgg
     1261 ggtcgtcgcc gtgccgggct accggcccgc ctacaacgac cgctggtacc tgcccgacga
     1321 tgccccggtc aacatcgggt tcgccgcggc ggaccggcat ttcacgcagg cgcgcatgaa
     1381 ccccgacatc ctcatggtcg aggccgacca cgacatgcgg aacccggccg acatgctggt
     1441 gctcgaccgc gtggccaaga acgtgctgcg cacgcagggc atcgcgatgg tccagagcat
     1501 cacgcgtcca ctcggaatcc ccatccagca cagctcgatt ccgttccaga ccgcgatcca
     1561 gggacagacc agcaacctga acctgccgtt ccagcgtgcg cagttggaca accagctcaa
     1621 gatggtcgag gcgacgaacc aatcgatcgc catcatggag aagcagtacg aactgtcgct
     1681 gcggcagacc aagctcacgc aggactcggc ggagaagtcg cgcgaactgc tcgaggtcac
     1741 caagcaactg cgcgacaaca tcgccaactt cgacgaccag ttccggccgt tgcgcaatta
     1801 cttctactgg gagccacact gtttcgacat cccgatgtgc gcggccgcgc gatcgttgtt
     1861 cgactccctc gacaccgtcg acgagttgac cgaccgaacg tcggcagtgc aggtcaacac
     1921 cgatgaattg gcggacctcg caccgcagtt gaccgctctg ctgccgcaga ccatcgcgtc
     1981 gatgaagacc agccgcgatc tgtcgctcgc gtcgtacaac tcgcagaagg ccctgctgga
     2041 ccagatgcag gccatgaacg acaccgcgct ggcgatgggg cagagcttcg acgaggcgaa
     2101 gaacgacgac ttcttctacc tgccgcccga ggcgttccag aacccggatt tcgaacgcgg
     2161 gctcaagatg ttcctgtcac cggacggcaa gtctgcacgc atgttcatca cccaccagag
     2221 cgatccggcg acaccggagg gcatcgcccg tgtgaacgcc gaacgcactg ccgcgcaaga
     2281 aggtctgaag atgtcgtcgc tgtcggacgc caagatcttc ctgggcggta ccgcggcgac
     2341 ctacaaggac atgagcgacg gtgcgcgcta cgacctgatg atcgcggtcg tgtcggcgct
     2401 gacgctgatc ttcatgatca tgctcatcct gacccgcagt gcggtggccg cgctggtcat
     2461 cgtgggcacc gcggcaagct cgatcgcggc gtcgttcggc atttcggtac tgctgtggca
     2521 ggacctgttc ggcgtggagg tgcactggct ggtcatgttg atgtcggtca tcatcctgct
     2581 ggccgtcggt tcggactaca acctgctgct cgtctcgcgc ttcaaggacg agatccacgc
     2641 ggggctgaaa accgggatca tccggtcgat ggccggcacg ggcggggtgg tcacctcggc
     2701 gggtctggtg ttcgccgcga ccatggccgc gatgctgggc agtgaactgg tggtgctcgc
     2761 gcagatgggg tcgaccatcg cgatcggtct gttgctcgac acgttgatcg tgcggtcact
     2821 gctcatgccg tcgatcgcga cgctgctcgg tcgctggttc tggtggccgc aggtggtcta
     2881 cccccgcggg gacaaccatt tccggcgacc caccgccgtc agccgcggca caggcgatga
     2941 ggacaccgca gcgctgcccg tgccgagcaa gcttggtgcg gccgcgggca acacgcctgg
     3001 catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg
     3061 tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc
     3121 caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc
     3181 ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa
     3241 gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat
     3301 ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt
     3361 caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga
     3421 gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc
     3481 gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa
     3541 gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa
     3601 cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct
     3661 cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa
     3721 ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg
     3781 ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt
     3841 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     3901 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     3961 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     4021 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     4081 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     4141 cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc
     4201 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     4261 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     4321 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     4381 tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     4441 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     4501 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     4561 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     4621 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     4681 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     4741 atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc
     4801 atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac
     4861 gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5041 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag
     5341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5641 cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga
     5701 ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag
     5761 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
     5821 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
     5881 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
     5941 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
     6001 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
     6061 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg
     6121 ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca
     6181 ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt
     6241 tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc
     6301 agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg
     6361 cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc
     6421 gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc
     6481 acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg
     6541 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
     6601 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
     6661 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
     6721 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
     6781 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
     6841 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
     6901 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
     6961 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
     7021 acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg
     7081 aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc
     7141 gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc
     7201 cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag
     7261 ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct
     7321 gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac
     7381 gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg
     7441 cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac
     7501 cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg gtgaccacaa
     7561 cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc acggccgtgg
     7621 gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc
     7681 tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt
     7741 ctctgacgag cgggagaact ccctatcag
//
