LOCUS       sequence-315658-        5447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0678:Dendra
FEATURES             Location/Qualifiers
     source          1..5447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1391..1414
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1671..2471
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2596..3184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3085..3104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgctgctttc cgatcgtgac atccgggccg agatcgccgc
      121 gaaacgcctg gccctcgagc cgttcgacga tgccctggtg cagccgtcga gcatcgatgt
      181 gcggctcgac cgcatgttcc gggtgttcaa caacacccgg tacacgcaca tcgaccccgc
      241 gatgcagcag gacgaactga ccacgctggt cgaacccgcc gagggcgagc cgttcgtcct
      301 gcacccgggg gagttcgtgc tggggtcgac gctggagctg tgcacgctgc ccgacgacct
      361 ggccggccgg ttggagggca agtcgtcact cgggcgtctg ggcctgctga cgcactcgac
      421 cgcgggcttc atcgatccgg gcttctccgg gcacatcacc ctggagctgt cgaacgtcgc
      481 gaacctgccc atcacattgt ggccgggcat gaagatcggg cagctgtgcc tgctgcggct
      541 caccagcccg gccgagaatc cgtacggcag cgccgcggtg gggtcgaaat accaggggca
      601 gcgtgggccg acgccgtcgc gctcgcacct caacttcatc aagtccaagc ttggtgcggc
      661 cgcgggcaac acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat
      721 ggagggcaac gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta
      781 cgaagggacc cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta
      841 cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga
      901 tatccctgac tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac
      961 cttcgaagac aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt
     1021 cttccagaac gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa
     1081 gaagaccctc aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt
     1141 cggcaacatc aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa
     1201 gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg
     1261 catcgaaatc ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt
     1321 ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct
     1381 gtatttccag gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc
     1441 taaacgggtc ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg
     1501 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     1561 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     1621 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
     1681 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
     1741 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1801 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg
     1861 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1921 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     1981 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     2041 tctctgatcc attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg
     2101 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     2161 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     2221 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     2281 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     2341 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     2401 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     2461 agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc
     2521 tgattgctgg ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa
     2581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2701 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3001 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3301 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg
     3361 gggatcctct agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg
     3421 cagccagctc gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg
     3481 ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag
     3541 ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg
     3601 tcagcatcga aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag
     3661 agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc
     3721 tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt
     3781 ctgccgacca catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
     3841 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
     3901 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
     3961 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
     4021 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
     4081 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
     4141 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
     4201 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
     4261 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
     4321 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
     4381 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
     4441 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
     4501 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
     4561 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
     4621 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
     4681 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     4741 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     4801 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt
     4861 cggccggaac tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca
     4921 ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg
     4981 aagtaccaga tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg
     5041 gccaagacct cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg
     5101 gggtttcttg tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct
     5161 gtgcggagac ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagaaa
     5221 tctagacggt gaccacaacg cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg
     5281 gcaccgtcac ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca
     5341 cggtgctgcg cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag
     5401 gcccggtgtg agaagggtct ctgacgagcg ggagaactcc ctatcag
//
