LOCUS       sequence-31566-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-PPI (PY00693)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     265..284
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     368..387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        368..386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    387..411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             426..448
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             467..484
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             497..517
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcn atgtgtgtcc
       61 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
      121 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
      181 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
      241 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
      301 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
      361 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      421 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
      481 tcacagcagc ggcagagaaa acttgtattt ccagggctat agtgatgacg aagaggagga
      541 aagtaatgct attaatgtag tttctgaaaa aacaaaatca ttggaagaaa aaattgctta
      601 ttataaaatg aaagggcata ctgaaagagg ctacataact atatatacaa atttaggaga
      661 ttttgaagta gaattatatt ggtaccatag tcctaaaaca tgtttaaatt tttatacatt
      721 atgtgaaatg ggtttctatg ataatacaat atttcataga gttataccta attttgttat
      781 tcaaggtggt gat
//
