LOCUS       sequence-315669-        5678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0132:Dendra
FEATURES             Location/Qualifiers
     source          1..5678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1622..1645
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1902..2702
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2827..3415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3316..3335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacggcgtc gaccgatggc ttcgtcgatt accttcgtgg
      121 ccagttggag aagccgctgg cgacggtcgg cggcttcttc aagatgtcgg tgatgaccgg
      181 aaaagctctg ttcacgcggc cttttcagtg gaaagagttc gtcctccaga gctggtttct
      241 catccgcgtg gccttcctgc cgacgcttgc ggtgtcgatc ccgctgacgg tgctcatcat
      301 cttcacgctg aacatcctgc tggcggagtt cggtgcggct gacgtctccg gggccggtgc
      361 cgcgctcggc gccgtgaccc agctaggtcc gctcgtgacg gtgctggtcg tcgcaggcgc
      421 cggatccacc gccatctgcg ccgatctcgg cgcccgcacg gtgcgtgagg agatcgacgc
      481 actcgaggtc ctcggcatcg accccatcga acgcctcgtc gtgccgcgcg tggtcgcctc
      541 gacgttcgtc gcgttcatgc tcaacggtgc ggtcatcaca atcggcctgg tcggtggatt
      601 cttcttcggt gtctatatcc agaacgtctc ggcgggcgct tacgtttcga cgctgaccct
      661 tctcacggga ttccccgagg tgttgatctc ggtcgtgaag gcgacgctgt tcggaatgat
      721 cgccggcctc gtcggctgct accgcgggct cacggtcgcg ggcggttcga agggtgtcgg
      781 caccgcggtg aacgagaccc tcgtgctatg tgtggtcgcg ttgttcgcag tcaacgtcgt
      841 gctcaccacc atcggtgtga ggttcgggac agggcgcaag cttggtgcgg ccgcgggcaa
      901 cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata tggagggcaa
      961 cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt acgaagggac
     1021 ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt acgatatcct
     1081 cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg atatccctga
     1141 ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga ccttcgaaga
     1201 caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt tcttccagaa
     1261 cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga agaagaccct
     1321 caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg tcggcaacat
     1381 caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca agaccacgta
     1441 caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc gcatcgaaat
     1501 cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg tggcacgcta
     1561 tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc tgtatttcca
     1621 ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct ctaaacgggt
     1681 cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac ggtaactgat
     1741 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
     1801 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
     1861 actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt
     1921 gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg
     1981 gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg
     2041 aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg ggagggacgc
     2101 tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg
     2161 ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat gtaaagcgca
     2221 gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc atctctgatc
     2281 cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg
     2341 ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat cttgccgagt
     2401 tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag gatggcaagt
     2461 tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc ttggcggaca
     2521 ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc tttgctcggt
     2581 tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga gatccgttga
     2641 tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg
     2701 gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg
     2761 gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     2821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2941 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3241 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3541 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     3601 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     3661 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     3721 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     3781 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     3841 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     3901 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     3961 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     4021 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     4081 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     4141 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     4201 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     4261 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     4321 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     4381 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     4441 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     4501 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     4561 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     4621 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     4681 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     4741 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     4801 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     4861 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     4921 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     4981 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     5041 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     5101 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     5161 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     5221 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     5281 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     5341 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     5401 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacgg
     5461 tgaccacaac gcgcccgctt tgatcgggga cgtcgaaata ttggatcgtc ggcaccgtca
     5521 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
     5581 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
     5641 gagaagggtc tctgacgagc gggagaactc cctatcag
//
