LOCUS       sequence-31567-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-FKBP-TPR
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(814..832)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
      181 agagaaaact tgtatttcca gggcgctaaa aaaagtatat atgattatac agacgaagaa
      241 aaagtacaat cagcttttga tataaaagaa gaaggaaatg aattttttaa aaaaaatgaa
      301 attaatgaag ccattgttaa atataaagaa gctcttgact tttttattca tactgaagaa
      361 tgggatgatc aaatattatt agataaaaaa aaaaatattg aaataagttg taatcttaat
      421 ctagccacat gttataataa aaataaagac tatccaaaag ctattgatca tgcatccaaa
      481 gtcttaaaaa ttgataaaaa taatgtaaaa gctttataca aattaggtgt tgctaatatg
      541 tactttggat tccttgaaga agcaaaagaa aatctttaca aagctgcatc attaaatcca
      601 aataatttag atataagaaa tagttatgaa ttatgtgtta acaaattaaa agaagctaga
      661 aaaaaagata aactaacttt tggaggcatg ttcgataaag gacctttata tgaagaaaaa
      721 aaaaatagtg caaattgatg acgaagcttg cggccgcact cgaggatccg gctgctaaca
      781 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      841 ttggggcctc taaacgggtc ttggaggggt tt
//
