LOCUS       sequence-31568-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pk-TCTP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(565..585)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(598..615)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             634..656
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    671..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(695..714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(696..714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(799..818)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgggcttgtt agcagccgga tcctcgagtg cggccgcaag cttcgtcatc aatacttttc
       61 ttcgtataat ccatctgata tataaacgaa acgtggagta acttcttcac ctttgtaata
      121 tgagtaagtc aagcctgcat ccatgtcaag tgattctccc atgtaaaatt caaagtcatc
      181 aaaatttgtc aaaatatgtt taatgagtgg ttgagctttc gtcttaaaaa cgtctacacg
      241 atctggtttc ttttcttcta aatatttgag aatcttttgc atatagttct ttatataaac
      301 actgtattcc tttttgctca aagatgtgga tgttaattgg aaagagtcaa caatatcaat
      361 aacttgctct acgtcggcac ccattccatc tactgcttct tcactattat cggcaatgcc
      421 atagtcgtca tttcctttta ttcttttatt tgatttcact tcaaaggcaa tttcgcgaaa
      481 gtctgcaatt ccaaatgggt cttcttgatt gtacgaatcg gaacatactt catcgttggt
      541 aaatacgtct ttgtatactt tcatgccctg gaaatacaag ttttctctgc cgctgctgtg
      601 atgatgatga tgatggctgc tgcccatggt atatctcctt cttaaagtta aacaaaatta
      661 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      721 cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      781 aggtgcggtt gctggcgccc tatatcgccg acatcaccga tgggggaaga tcgggctcgc
      841 cacttcgggc tcatgagcgc ttgnttcggc gtgggtatgg tggcaggccc cgtggccggg
      901 ggactggttg gggcgccatc tccttgcatg
//
