LOCUS       sequence-315680-        7760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0381:Dendra
FEATURES             Location/Qualifiers
     source          1..7760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3704..3727
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3984..4784
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4909..5497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5398..5417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaccgaacc agcgaccacc cagaaccgcg aggcccgacc
      121 caggatcgcc tcgttcatcc gccgattttc cgtactgatc atcctggcgt ggatcgttgt
      181 ggcggcactg gctacctttg cgatcccgcc actcgaagtc gtggaacgtg agcactcggt
      241 atcgctgagc ccgccggatg cgccctcggt ccaggcgatg aagcggatgg gcgaggtctt
      301 tcaggagtcg aactccaaca gcgttgcggt cttggtcctg gagggtcagg aacccctcgg
      361 cgacgacgcg catcggtact acgacgagat catccggaag ctcaaggacg atccggagca
      421 cgtccagcac gtccaggact tctggggtga cccgctcacc gccggtgccg ccgagagcgg
      481 tgaccgcaag gctgcctacg ttcagatcaa tctgcatggg agtttcggcc aggcagaggc
      541 caacgaatcc gtcaaggccg tccaggacgc ggtcgagagc acgccggcgc ctccaggagt
      601 tcaggcattc gtcacgggcc ccgcggcgat tgtcgccgac atgggcgaga gcggtaacaa
      661 gacggttctg ctgatcaccc tcgtcaccgt cggtgtcatc ttcctgatgc tgctcctgct
      721 ctatcgcagg gtgctcatcg tcttgatcct gctggcgacc gtcgcgatcg agttgcaggt
      781 ggctcgcggc ctggtttcct tcctggcgtt gaacggcatc gtcggcctga ccacctacgt
      841 ggtgaacctg ctggtatcgg tcggcatcgc cgctggtacc gactatggca tcttcttcgc
      901 gggtcgttac caggaggcac gtcaagcggg cgaggatcgg gagcaggcgt actacacgtc
      961 gttcgcgggc gtggcaaagg tcgtcctggc ctccggtgcc acgatcgcgg gcgcgatcgc
     1021 gtgcctcagt ttcactcggc tgccctactt ccagcctctc ggtatccccg gtgctgtggg
     1081 tatcggcgtc gccgtcgcgg ttgctctgac actggtgccc gcctgcatcg cggcgaccaa
     1141 ccgtttcggc tggttcgacc cgagacgtct ggtgaccaca cgccgttggc ggcgcatcgg
     1201 taccgccgtg gtgcgttggc ctgcaccgat tttgatcgcc accatcgccg tggcggtgat
     1261 aggcctgctg actctacccg gctacaaccc gagctacagc gatcagaagt acatcccgca
     1321 ggacatcccc gcgaaccagg gattcgcggc ggccgcgcgc cacttcccgg agtcgaagat
     1381 gacgacgccc gacatcctgc tggtcgaagc cgaccacgac atgcgtaatt cggctgatct
     1441 gcttgtgttg aacaagttgg ccaaagccgt gttcgcggtt cccggtgtgt cgaacgtcca
     1501 ggcgatcacc aggccagagg gaacgccgat cgagcacacc tcgattccct tcatgttgag
     1561 catgtccaac gccagtcaga gactgagcct gccgttccag caacagcgca tggaagacct
     1621 gctcaagcag gccgacgagc tgacgaagaa catcgcgttg atgaagcaca tgtacgcgtt
     1681 gatgcaggag atggtggcca ccacccacag cacagttgaa acgacacacg aggttcagca
     1741 gaccacgaag gatctgcggg acagcatcga ggatttccaa gacttctggc ggccgatccg
     1801 caactacctg tactgggaac cgcactgcta cgacatcccg ctgtgctggt ctatccgatc
     1861 gctgttcgac ggactcgacg gcatcaacga ggtcaccgac aagatgggcg atctggtcga
     1921 ggatctcgac cacttggaca cgctcatgcc gcagatgttg ctgcagtttc cgccgatgat
     1981 cgcaatgctg gagaacagcc ggtcgatgat cctgacaatg cacagcacga tgtcgggcat
     2041 cttctcgcag atggacgact ccaccgacaa cgcgacggcg atggggaagg cgttcgacgc
     2101 cgcgcgcaac gacgactcgt tctaccttcc gccggacatt ctcgaaaatg aagacttcaa
     2161 acgcgtgatg aagatcttca tgtcgcctga cggcaaggcg gcgcgcatgt tgatcaccca
     2221 acgtggtgat ccggcgacgc cggagggtat tgcgttggtc gagcccatcc gggtggcggc
     2281 agaagaagcc ctcaagggca caccgctgga aagtgcagag ttgcatctcg ccgggaccgc
     2341 agcgggggtc aaggatctgg tcgacgggtc gaagatcgac ctgctgatcg cgggtgtcgc
     2401 tgcgctttgc ctgatcttca tcatcatgct gatcatgacg cggagcttca tcgccgcgct
     2461 ggtcatcgtg ggcaccgtag cgatgtcgct gggcgcatcg ttcgggcttt cggtgctggt
     2521 gtggcagcat ctgctgggca tccagatcaa ctgggtcgtg ctggcgatgt cggtcatcgt
     2581 tctcctggca gtcgggtcgg actacaacct gctgctcgtg tcccggatga aagaagagat
     2641 aggcgccggc ttgaacaccg gcatcatccg agcaatgggc ggaaccggaa aggtcgtcac
     2701 cgcggcaggt ctggtcttcg cagccacgat ggcgtcgatg atcgtcagtg atctgctcac
     2761 catcggtcag gtcggcacga cgatcggtct gggtctgctg ttcgacacct tggtcgtgcg
     2821 tgctttcatg acaccgtcca tcgcagccct tctggggcgt tggttctggt ggccgcagcg
     2881 tgtgcgtcag cgtcccgcca gcgcgatgct ccgctccgtc ggcccacgcc ccctggtgcg
     2941 atccctgtta ctgagggata agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
     3001 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     3061 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     3121 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     3181 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     3241 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     3301 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     3361 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     3421 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     3481 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     3541 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     3601 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     3661 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     3721 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     3781 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     3841 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     3901 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     3961 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     4021 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     4081 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     4141 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     4201 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     4261 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     4321 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     4381 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     4441 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     4501 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     4561 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     4621 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     4681 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     4741 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     4801 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     4861 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5041 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5641 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     5701 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     5761 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     5821 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     5881 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     5941 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     6001 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     6061 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     6121 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     6181 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     6241 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     6301 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     6361 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     6421 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     6481 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     6541 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     6601 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     6661 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     6721 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     6781 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     6841 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     6901 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     6961 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     7021 cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc
     7081 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc
     7141 aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc
     7201 cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc
     7261 cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc
     7321 gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc
     7381 ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc
     7441 acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc
     7501 cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc
     7561 tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg ggaggcggca
     7621 cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca
     7681 tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg tctctgacga
     7741 gcgggagaac tccctatcag
//
