LOCUS       sequence-315687-        6032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0003:Dendra
FEATURES             Location/Qualifiers
     source          1..6032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1976..1999
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2256..3056
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3181..3769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3670..3689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgtttgcccg gcatctcgga ctgaccgatt tccggtcgtg
      121 ggcacgggtc gatctcgatc tcgagccggg gcgcaccgtc ttcgtgggcc ccaacggctt
      181 cgggaagacg aatcttgttg aggcgctgtg gtattcggca acgctcggat ctcatcgggt
      241 ggccaccgac gcgccgctga tccgtgcggg tgccgaacgc gcgatcgtgt cgaccatcgt
      301 cgtcaacgag ggtcgcgaac tcgcggtgga tctcgagatc accagtggcc gggccaacaa
      361 ggccaggttg aaccgttccc cggtacgcag tgcccgcgag atcctcgggg tgctgcgcgc
      421 ggtgttgttc agccccgagg atctctcgct ggtgcgcggg gatccgggtg accgcaggcg
      481 ttacctcgac gaactcgcga ccacgcggcg tccggcgctc gcgggtgtgc gcgcggatta
      541 cgacaaggtg gtccgccagc gcacggcctt gctgaaaacc gctgcgggag cacgttatcg
      601 gggcgaccgt agcgtgatcg acacgctcga ggtgtgggac gggcacctcg ccgcgcacgg
      661 cgcggcgctc gtggcgtcgc gcgtgaaact cgtcgaggaa ctgcagccag aggtcgagaa
      721 ggcctaccag ttgctcgcac cggcctcgcg tcccgcggcc atccggtacc gcagcagtgt
      781 cgaggccata gaggacgcgc cgggccccga gagcgtggag ttctacgagg ccgcactgct
      841 cgacgcactg gcgcgccgcc gtgatgccga gctcgagcgt ggtgtgtgcc tcgtcgggcc
      901 gcaccgcgac gacctggaac tgcgtctcgg tgaccagccc gcgaaaggct ttgccagcca
      961 tggggaatcg tggtcgatgg cgctggcact gcgactcggc gcctatgaac tgttgtgctc
     1021 cgacggcgtc gagccggtgc tgctcctcga cgacgtgttc gccgaactgg acacctcgag
     1081 gcggcgcgcg ctggccaccg tggccggctc ggccgagcag gttctggtga ctgccgctgt
     1141 gggcgaggac attccggagg actgggacgc gcgtcgcgtc gagatccgga tggtcgagga
     1201 cgacggcgga cgcgtttcga tggtggcctc aaagcttggt gcggccgcgg gcaacacgcc
     1261 tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg catatggagg gcaacgtgaa
     1321 cggtcacgcc ttcgtgatcg agggggaggg taagggtaag ccttacgaag ggacccagac
     1381 cgccaacctc accgtcaagg agggcgcccc tctcccattc tcgtacgata tcctcactac
     1441 cgcggtgcat tacgggaacc gcgtgttcac caagtacccc gaggatatcc ctgactactt
     1501 caagcagagc ttcccggagg gatactcctg ggagcggacg atgaccttcg aagacaaggg
     1561 tatctgtacg atccgcagcg acatctcgct ggaaggggac tgtttcttcc agaacgtccg
     1621 tttcaagggg accaactttc cgccgaacgg cccggtcatg cagaagaaga ccctcaagtg
     1681 ggagccgagc acggagaagc tgcacgttcg ggacggcctc ctggtcggca acatcaacat
     1741 ggcgctgctg ctggaaggcg gcggccacta cctatgcgac ttcaagacca cgtacaaagc
     1801 gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac caccgcatcg aaatcctggg
     1861 gaacgattcg gactacaaca aggtcaagtt gtacgagcac gctgtggcac gctatagccc
     1921 gctcccgtcg caggtctggt tcgaaggcgg cggcggggag aacctgtatt tccaggacta
     1981 caaggacgac gacgacaagt gatttaaata accccttggg gcctctaaac gggtcttgag
     2041 gggttttttg ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac tgatgccgta
     2101 tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa
     2161 tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt
     2221 tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg
     2281 tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta
     2341 cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg
     2401 ggccacttgg actgatcgag gccctgcgtg ctacgctggg tccgggaggg acgctcgtca
     2461 tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac
     2521 cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc
     2581 atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc
     2641 ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg
     2701 tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg
     2761 caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac
     2821 gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc
     2881 tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc
     2941 gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc
     3001 tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag
     3061 ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt gctggggcaa
     3121 cacgggggat ccactagttc catgagcgtc agaccccgta gaaaagatca aaggatcttc
     3181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3301 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaggg
     3601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3901 ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt acccggggat cctctagagt
     3961 cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc agctcgctga
     4021 gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa
     4081 ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg
     4141 aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta
     4201 gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt
     4261 gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg
     4321 gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat
     4381 gggccggtca agataggttt ttaccccctc tcggctgcat cctctaagtg gaaagaaatt
     4441 gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag gagttgcaac ccggtagcct
     4501 tgttcacgac gagaggagac ctagttggca cgtcgcggat ggggatcgct gaagactcag
     4561 cgcagcggga ggatccaagc ctcatacgtc aacccgcagg acggtgtgag gtactacgcg
     4621 ctgcagacct acgacaacaa gatggacgcc gaagcctggc tcgcgggcga gaagcggctc
     4681 atcgagatgg agacctggac ccctccacag gaccgggcga agaaggcagc cgccagcgcc
     4741 atcacgctgg aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc agacggcacc
     4801 agggatctgt acagcgggca cgcggagcgc cgcatctacc cggtgctagg tgaagtggcg
     4861 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
     4921 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
     4981 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
     5041 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
     5101 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
     5161 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
     5221 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
     5281 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
     5341 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
     5401 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
     5461 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
     5521 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
     5581 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
     5641 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
     5701 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
     5761 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
     5821 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     5881 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     5941 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     6001 ggtctctgac gagcgggaga actccctatc ag
//
