LOCUS       sequence-315689-        5804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0156:Dendra
FEATURES             Location/Qualifiers
     source          1..5804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1748..1771
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2028..2828
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2953..3541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3442..3461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgctgttgcg ccagttggag tacttcgtcg ccgtggcccg
      121 ggaacgtcac ttcgccaggg ctgccgaggt ctgctacgtg tcgcaaccgg cgctgtccac
      181 agcaattgcc aagctcgagc gcgagctcga cgtcacgctg atccaccgcg gccagaactt
      241 cgagggcctc acgctcgaag gcgaacgtct cgtggtgtgg gccaagaggt tgctggccga
      301 gcacgacggc ctcaaggccg aggcggccgc gttgcgcacc ggcatctccg gaacgctacg
      361 gctgggcacc gggcccacgg tgtcgacgac gacggccatc cccgttgcgg cgttctgcaa
      421 cctgcacccg aaggccaacg tctccatatc ctcccggctg tcctcggccg aattgctgcg
      481 acagctccgc gatttcgaac tcgacgcggc catcgcccat ttcgggccgg aggaccgagc
      541 cgggctcgag gtggtgccgt tgtacaagga gcggtacgtg ctgctggtct ccggagatca
      601 gctggcaccc ggtacgcgcg ccatcacgtg gtccgacgcc gcgcagttgc cgctggcgct
      661 gctcgagccg cacatgcggt tccggcagtt catcgacaag gccttcgccg agaacggcgt
      721 gacgccgtcg ccgcaggtcg aaacggattc ggtggcatcg ctgtatgccc tggtggccac
      781 cggcgcctgg gcgagcgtcg tgccccacac gtggctgcgg gccgtccccc agctgggagc
      841 ggccagagcc gtgcggctca tcgaaccaga gaccacagca cagatctcgc tcgcgatcca
      901 cgccggccgc ggctccgcgg ccgcgcgcgc cttcgtcaat gtcgccgcag gcgcccgcct
      961 ggacgatcaa ttcgatctgc agtcagaaca ctggctgctc aagaagcttg gtgcggccgc
     1021 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1081 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     1141 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     1201 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     1261 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1321 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     1381 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     1441 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     1501 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     1561 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     1621 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     1681 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     1741 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     1801 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     1861 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     1921 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     1981 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     2041 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     2101 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     2161 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     2221 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     2281 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     2341 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     2401 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     2461 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     2521 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     2581 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     2641 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     2701 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     2761 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     2821 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     2881 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     2941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3061 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3301 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3361 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3421 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3481 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3541 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3601 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3661 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     3721 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     3781 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     3841 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     3901 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     3961 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     4021 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4081 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     4141 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     4201 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     4261 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4321 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     4381 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     4441 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     4501 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     4561 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     4621 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     4681 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     4741 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     4801 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     4861 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     4921 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     4981 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     5041 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     5101 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     5161 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     5221 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     5281 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     5341 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     5401 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     5461 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     5521 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     5581 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     5641 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     5701 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     5761 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
