LOCUS       sequence-315692-        6136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0030:Dendra
FEATURES             Location/Qualifiers
     source          1..6136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2080..2103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2360..3160
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3285..3873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3774..3793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccattt gagcccgcgc gttggagtca cgctgtccgg tcgttaccgg
      121 ctgcagcggc tgatcgccac gggcggtatg ggccaggtgt gggaggccgt ggattcgcgg
      181 ctgggccgcc gggtcgccgt caaggtgctc aaggccgagt tctccaccga cgccgagttc
      241 gtcgaacgct tccgggccga ggcccgcacc gtcgcgatgc tcaaccatcc cggcatcgcg
      301 agcgtgtacg actacggcga gaccgaactc gacggcgagg gccgcaccgc gtacctggtg
      361 atggagctca tcaacggtga gccgctgaac tcggtgctca agcgcaccgg ccggctgtcg
      421 ctgcggcacg cgctggacat gctggagcag accggccgtg cgctgcaggt cgcgcacacc
      481 gcgggcctgg tgcaccgcga cgtcaagccc ggcaacatcc tcatcacccc gacggggcag
      541 gtgaagctca ccgacttcgg catcgccaag gccgtcgacg ccgcgccggt cacccagacc
      601 ggcatggtga tgggcaccgc gcagtacatc gcgcccgagc aggccctggg ccatgacgcg
      661 accgccgcca gtgacgtgta ttcgctgggc gtcgttggct acgaatcggt ttcgggcaag
      721 cgcccgttca ccggcgacgg tgcgctgacg gtcgcaatga agcacatcaa ggagaccccg
      781 ccgccgcttc cggccgatct gccgccgaac gtgcgcgagc tgatcgagat cacgctcgtg
      841 aagaacccgg gcatgcggta caagtcgggc ggcccgttcg ccgacgcggt cgccgcggtg
      901 cgcgcgggtc gcaggccacc gcggcccaac caggcaccga cgctgggacg tgccgcaccg
      961 gccgcggtgc cgtcggccgc gcaggcccgt gcctctgccg atctcacggg ccgtgcgccc
     1021 gtcaccgccg cacgcgcgag acccaccgcg acggccgcgc atcggactcc gccgcctcga
     1081 cgcacgttct cctcggggca acgggcgcta ttgtgggcgg ctggcgtcct cggcgcgttg
     1141 gccatcgtga tcgcgatcct gatcgtgctc aacgcgcagg accgcaagga ccgcgagcag
     1201 tcgccaccgc cgacggtcac cgacaccgtc accgagacga cgccctacga ggagaccccg
     1261 gcggcgatgc ctgacctcat gatcatgttg cgtgcggctc agcccgagcc gccgccatcc
     1321 gaacagatac agcgaaagct tggtgcggcc gcgggcaaca cgcctggcat caatctgatc
     1381 aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg
     1441 atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa cctcaccgtc
     1501 aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt gcattacggg
     1561 aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca gagcttcccg
     1621 gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg tacgatccgc
     1681 agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa ggggaccaac
     1741 tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc gagcacggag
     1801 aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct gctgctggaa
     1861 ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa ggtcgtccag
     1921 ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga ttcggactac
     1981 aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc
     2041 tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga cgacgacgac
     2101 aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt tttgttaact
     2161 agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt
     2221 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
     2281 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgccgttg
     2341 atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg
     2401 gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg gcggtgtgct
     2461 gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac ttggactgat
     2521 cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct cgtggtcagg
     2581 tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc ttggagttgt
     2641 ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc
     2701 ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc caccttactc
     2761 gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc tcctcggcgt
     2821 gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg ttccctatgg
     2881 ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg attatctcga
     2941 gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg agaagagcct
     3001 tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc gcgacattgt
     3061 ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc cgccagaggc
     3121 gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg agcggagaac
     3181 gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg ggatccacta
     3241 gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3421 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa ggcggacagg
     3721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4021 gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca ccaagggcac
     4081 catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg tgaacgacag
     4141 ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg gtgcaacctt
     4201 gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt
     4261 tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga tcagggatgc
     4321 gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac ccctggaaag
     4381 aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg ggaatcgaac
     4441 ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg gtcaagatag
     4501 gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt cgtagaagcg
     4561 cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca cgacgagagg
     4621 agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc gggaggatcc
     4681 aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag acctacgaca
     4741 acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag atggagacct
     4801 ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg ctggaggagt
     4861 acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat ctgtacagcg
     4921 ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca gagatgacgc
     4981 cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact gcccgccggc
     5041 atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag ctgatcgcag
     5101 agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc
     5161 ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc gcggcataca
     5221 tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc aaggacatcg
     5281 tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca
     5341 agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc
     5401 acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac aagggccccg
     5461 aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg ttcaccaagt
     5521 cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac gacctccgcg
     5581 ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg atggcccgtc
     5641 tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag gcccgcgacg
     5701 aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca aaaagccccc
     5761 ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga agaaccacgc
     5821 ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag ccccgccgcc
     5881 gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc gcccgctttg
     5941 atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
     6001 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
     6061 ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg
     6121 gagaactccc tatcag
//
