LOCUS       sequence-31571-e         187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 187)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-KsgA
FEATURES             Location/Qualifiers
     source          1..187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggccagca tcttttaaaa aacccaggta
      121 ttttagataa aattatatat gcagcaaaaa taaaaagctc agatatagta ttagaaattg
      181 gatgtgg
//
