LOCUS       sequence-31572-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pv-2Cys-Prx
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     267..286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     370..389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        370..388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             429..451
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             470..487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             497..514
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gactctcttc cgggcgctat catgccatac cgcgaaaggn tttgcgccat tcgatggtgt
       61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
      121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataaacaa ttcccctcta
      421 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
      481 tcatcacagc agcggcctgg ttccgcgtgg tagtccgaca tacgtaggaa aggaagcccc
      541 ctttttcaaa gctgaggccg tttttggaga taactccttt ggagaagtga atttaacaca
      601 atttatcgga aagaagtatg tgctgttata tttttaccct cttgatttta ccttcgtgtg
      661 cccatcagaa ataatagcat tggataaagc cttggatgca tttcacgaaa ggaatgtaga
      721 attgttggga tgcagtgtag atagcaaata cacacacctt gcatg
//
