LOCUS       sequence-315726-        6713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0406:Dendra
FEATURES             Location/Qualifiers
     source          1..6713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      557..566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2657..2680
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2937..3737
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3862..4450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4351..4370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagccacta caagagcaat gtccgtgatc aggtattcaa
      121 cctgttcgag gttttcggtg tggacaaggt gctcggcgct gacaagttca gcgacctgga
      181 tgccgacacc gcccgcgaga tgctcaccga gatcgcgcgc ctggccgagg gcccgatcgc
      241 cgaatccttc gtcgagggtg accgcaatcc cccggtgttc gatcccgaaa cccataccgt
      301 cacgctgccc gagggcttca agaagtcgat gcgtgcgctg ttcgacggcg gctgggacaa
      361 ggtcggcctc gcagagcacc tcggcggcat cccgatgccg cgtgcactgc agtgggccct
      421 gatcgagcac atcctcggcg ccaaccctgc ggcctacatg tacgccatgg gcccgggcat
      481 gagcgagatc ttctacaaca acggcaccga cgagcagaag aagtgggcca ccatcgccgc
      541 cgagcgcggc tggggcgcca ccatggtgct gaccgaaccc gacgcgggct ccgacgtcgg
      601 cgcgggccgc accaaggcag ttcagcagcc tgacggcacc tggcacatcg agggtgtgaa
      661 gcgattcatc acctcggccg actccgacga cctgttcgag aacatcatgc acctggtgct
      721 ggcccgcccc gagggcgccg gaccgggcac caagggtctg tcgctgttct tcgtgccgaa
      781 gttccacttc gaccacgaga ccggtgagat cggtgagcgc aacggcgttt tcgtcaccaa
      841 cgtcgagcac aagatgggcc tgaaggtctc ggccacgtgt gagctgtcgc tgggtcagca
      901 cggcatcccc gccgtcggct ggctggtggg cgaggtgcac aacggcatcg cgcagatgtt
      961 cgacgtgatc gagcaggcgc gaatgatggt gggtaccaag gccatcgcca cgctgtcgac
     1021 cggttacctc aatgcgctcg agtacgccaa ggagcgtgtc cagggcgccg acatgacgca
     1081 gatgaccgac aagaccgcac cgcgcgtgac catcacgcac cacccggatg tgcgtcgcag
     1141 cctgatgacc cagaaggcct acgccgaggg tctgcgtgcg atctacctgt acaccgcgac
     1201 cttccaggac gccgaggtgg cccaggccgt gcacggcgtc gacggcgacc tcgccgcacg
     1261 cgtcaacgat ctgctgctgc cgatcgtcaa gggcttcggc tcggagaccg cctacgccaa
     1321 gctcaccgag agcctgcaga ccctcggtgg ttccggcttc ctgcaggact acccgatcga
     1381 gcagtacatc cgcgactcga agatcgactc gctgtacgag ggcaccactg ccatccaggc
     1441 gcaggacttc ttcttccgca agatcatccg tgacaagggt caggcactgg cctacgtcgc
     1501 gggcgagatc gagcagttca tcaagaacga gaacggcaac ggccgcctga agaccgagcg
     1561 cgaactgctc gccaccgcgc tcgccgacgt ccagggcatg gccgcttcac tgaccggcta
     1621 cctgatggcc gcgcaggagg atgccgcctc gatctacaag gtgggcctcg gctcggtccg
     1681 gttcctgatg gcggtcggcg atctgctgtc cggctggctg ctggcacgcc aggccgcggt
     1741 ggccatcgag aagctcgacg caggcgccac cggtgccgac aagtccttct acgagggcaa
     1801 gatcgccgcg gcgtcgttct tcgccaagaa catgctgccg ctgctgacca gcacgcgcca
     1861 gatcatcgag aacctcgaca acgacgtcat ggagttggac gaggcggcgt tcaagcttgg
     1921 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1981 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     2041 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     2101 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     2161 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     2221 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     2281 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     2341 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     2401 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     2461 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     2521 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     2581 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     2641 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2701 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2761 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2821 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2881 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2941 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     3001 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     3061 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     3121 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     3181 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     3241 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     3301 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     3361 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     3421 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     3481 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3541 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3601 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3661 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3721 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3781 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4021 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4081 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4141 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4201 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4261 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4321 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4441 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4501 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4561 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     4621 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4681 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4741 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4801 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4861 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4921 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4981 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     5041 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     5101 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     5161 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     5221 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     5281 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     5341 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     5401 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     5461 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     5521 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     5581 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     5641 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5701 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5761 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5821 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5881 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5941 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     6001 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     6061 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     6121 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     6181 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     6241 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     6301 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     6361 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     6421 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     6481 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     6541 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     6601 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     6661 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
