LOCUS       sequence-31573-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pv-ASL
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggttccgcgt ggtagtgagc acctgaagaa catctcgccc atcgacgggc
      121 ggtacaaaaa ggcgtgcggc gaactgtcgg cgttcttctc cgagcacgcg ttgataaagc
      181 accggatcat cgtcgaggtg cggtggcttc tcttcctcaa cgaggaggag ctcttcttcg
      241 aaaaggtgac cgaccactcg gtggaggtgc tgaaccagat cgccacaaac ataacggaca
      301 gcgacattgc aagagtgaaa gcgatagagg aggaaaccaa ccacgatgta aaggcagtag
      361 aatattttgt gaaggaaaaa ttgaaaaact caaaaaggga agacctccta aagataaaag
      421 aatacgtaca ctacctctgc acaagtgaag atattaacaa tgtagcttat gccacctgtc
      481 tgaaggcatg cctaaacgat gtagtaatcc cctgcttgga aaaaataatg ctcaagctga
      541 aggacctagc tgtggagtac tcccatgtgc cgctactctc aaggacacat ggccaacctg
      601 catcttccac cacattcgga aaagaaatgg ctaatttcta cgcgaggata caccaccatg
      661 tgggggtcat tagaagagtg aaggtatgtg caaagtttaa cggagctgtg gggaatttca
      721 atgcgcataa agtggccagc aaagacaccg actgggtgaa taccattgga ttatttttaa
      781 aaaagcattt caacttaaca tacagcatat attgcacgca gatacaagac catgattaca
      841 tatgcgagct gtgtgatggt ctggctagag caaacggtac tctgattgac ctctgcgtag
      901 atatctggtt atacatttct aataatttac taaagctc
//
