LOCUS       sequence-315733-        6224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0581:Dendra
FEATURES             Location/Qualifiers
     source          1..6224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2168..2191
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2448..3248
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3373..3961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3862..3881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaccatcgc cgagatcgcc ggtcggacac tcacccagga
      121 acgccggctc gtcaccgcca ttcccggccc gatctcgcag gagctgcagg cgcgcaagca
      181 gagtgccgtg gcggcgggtg tcggcgtgac gctgccggtc tacgtcgtcg cggccggtgg
      241 cggcgtgctc gcggacgccg acggcaacca gctcatcgac ttcggctcgg gcatcgccgt
      301 caccaccgtc ggcaacagcg cacccgccgt cgtcgacgcg gtgacgcagc aggtcgcagc
      361 gttcacccac acgtgcttca tggtcacgcc ctacgaggga tacgtgaagg tggccgagca
      421 cctcaaccgg ctcaccccgg gcgatcacga gaagcgcacg gcgctgttca actccggcgc
      481 cgaagccgtc gagaacgcgg tcaagatcgc gcgtgcctac acccgcaggc aggcggtggt
      541 ggtgttcgac cacgcctacc acggccgcac caacctgacc atggcgatga ccgccaagaa
      601 ccagccgtac aagcacggct tcggcccgtt cgccaacgag gtgtaccgcg tgcccacgtc
      661 gtacccgttc cgcgacggcg agaccgacgg cgcggccgcc gcggcgcacg ccctcgacct
      721 gatcaacaag caggtcggcg ccgacaacgt cgccgccgtg gtgatcgaac ccgtccacgg
      781 tgagggcgga tttgtcgttc ccgcaccggg attcctcggc gctctgcaga agtggtgcac
      841 cgacaacggc gcggtgttcg tcgccgacga ggtgcagacc ggattcgccc gcaccggtgc
      901 gctgttcgcg tgcgagcacg agaacgtggt gcccgatctg atcgtcaccg ccaagggcat
      961 cgcaggcggt ctgccgctgt cggcggtgac cggtcgcgcc gagatcatgg acggcccgca
     1021 gtccggtggc ctcggcggca cctacggcgg caacccgctg gcgtgtgccg ccgcgctcgc
     1081 ggtgatcgac accatcgagc gtgaaaacct cgtcgcgcgg gcccgcgcga tcggcgagac
     1141 catgctgagc cgcctcggtg cgctcgccgc agccgatccg cgcatcggcg aggtgcgtgg
     1201 ccgcggcgcc atgatcgctg tcgaactggt caagccgggc accaccgagc ccgacgccga
     1261 cctgaccaag cgcgtcgccg ccgctgcgca cgcgcagggc ctggtggtgc tgacgtgcgg
     1321 cacctacggc aacgtgctgc gcttcctgcc cccgctgagc atgcccgacc acctgctcga
     1381 cgaaggactc gacatcctgg ccgcagtgtt cgcagaggtg aagaagcttg gtgcggccgc
     1441 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1501 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     1561 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     1621 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     1681 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1741 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     1801 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     1861 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     1921 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     1981 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     2041 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     2101 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     2161 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     2221 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     2281 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     2341 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     2401 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     2461 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     2521 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     2581 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     2641 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     2701 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     2761 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     2821 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     2881 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     2941 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     3001 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     3061 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     3121 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     3181 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     3241 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     3301 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     3361 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3421 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3481 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3541 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3601 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3661 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3721 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3781 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3841 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3901 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3961 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4021 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4081 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     4141 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     4201 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     4261 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     4321 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     4381 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     4441 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4501 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     4561 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     4621 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     4681 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4741 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     4801 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     4861 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     4921 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     4981 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     5041 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     5101 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     5161 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     5221 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     5281 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     5341 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     5401 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     5461 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     5521 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     5581 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     5641 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     5701 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     5761 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     5821 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     5881 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     5941 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     6001 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     6061 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     6121 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     6181 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
