LOCUS       sequence-315735-        6152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0688:Dendra
FEATURES             Location/Qualifiers
     source          1..6152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2096..2119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2376..3176
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3301..3889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3790..3809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggaacgcgt gactacccac cagttgccgt ggcagagcac
      121 cggggaacac cggaagccgc gcacgttcac ccagtccacg aagctgcagg acgtcctgta
      181 cgagatccgt gggccggtac acgagcacgc ctcacggctg gaagctgaag ggcatcgcat
      241 cctcaagctg aacatcggca accccgcgcc gttcgggttc gaggcccccg acgtcatcat
      301 gcgggacatc atccaggccc tgccgaccgc gcagggctat tcagactcca agggaatcct
      361 cagcgcccgg cgcgccgttt tcacgcgcta cgagctggtc gagggcttcc ccaagttcga
      421 cgtcgacgac gtctacctgg gcaacggcgt ctccgagctc atcaccatga cgctgcaggc
      481 gctgctggac aacggcgacc aggtgctcat cccggcaccc gactacccgc tgtggacggc
      541 gtcgacctcg ctggccggcg gcaccccggt acactacctg tgcgacgaga cgcagggctg
      601 gaaccccgac gtcgccgaca tcgaatcgaa gatcaccgaa cgcaccaagg cgatcgtggt
      661 gatcaacccg aacaacccga cgggcgcggt gtacagccgc gaaaccctcg agcagatcgt
      721 cgacctggcg cgcaaacatc agctgctgct gctggccgac gagatctacg acaagatcct
      781 ctacgacgac gccaagcaca tctcactggc gacgttggcg cctgacctac tctgtttgac
      841 tttcaacggg ctgtcgaagg cctaccgggt ggccggctac cggtcggggt ggctggtgat
      901 caccggcccc aaggagcacg ccaccagttt catcgagggc atcagcctgc tggccaacat
      961 gcgcctgtgc ccgaatgtcc ccgcgcagca cgccatccag gtggccctgg gcggacacca
     1021 gagcatcgac gcgttggtgc tgcccggcgg gcggctgctc gaacagcgtg acgtggcgtg
     1081 ggagaagctc aacgagatcc ccggggtgtc gtgcgtgaag ccgcgcggtg cgctgtacgc
     1141 gttcccccgg ttggaccccg aggtgtacga catccacgac gacgagcagc tggtcctcga
     1201 cctgctgctg caggagaaga tcctgctgac ccagggcacg gggttcaact ggcccacacc
     1261 cgatcacctg cgcatcgtga cgttgccgtg ggcgcgcgat ctggcctcgg cgatcgagcg
     1321 gctggggaac ttcctggcga gctaccgtca gaagcttggt gcggccgcgg gcaacacgcc
     1381 tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg catatggagg gcaacgtgaa
     1441 cggtcacgcc ttcgtgatcg agggggaggg taagggtaag ccttacgaag ggacccagac
     1501 cgccaacctc accgtcaagg agggcgcccc tctcccattc tcgtacgata tcctcactac
     1561 cgcggtgcat tacgggaacc gcgtgttcac caagtacccc gaggatatcc ctgactactt
     1621 caagcagagc ttcccggagg gatactcctg ggagcggacg atgaccttcg aagacaaggg
     1681 tatctgtacg atccgcagcg acatctcgct ggaaggggac tgtttcttcc agaacgtccg
     1741 tttcaagggg accaactttc cgccgaacgg cccggtcatg cagaagaaga ccctcaagtg
     1801 ggagccgagc acggagaagc tgcacgttcg ggacggcctc ctggtcggca acatcaacat
     1861 ggcgctgctg ctggaaggcg gcggccacta cctatgcgac ttcaagacca cgtacaaagc
     1921 gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac caccgcatcg aaatcctggg
     1981 gaacgattcg gactacaaca aggtcaagtt gtacgagcac gctgtggcac gctatagccc
     2041 gctcccgtcg caggtctggt tcgaaggcgg cggcggggag aacctgtatt tccaggacta
     2101 caaggacgac gacgacaagt gatttaaata accccttggg gcctctaaac gggtcttgag
     2161 gggttttttg ttaactagcg tacgatcgaa ttcctgcagc tcacggtaac tgatgccgta
     2221 tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa
     2281 tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt
     2341 tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg
     2401 tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta
     2461 cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg
     2521 ggccacttgg actgatcgag gccctgcgtg ctacgctggg tccgggaggg acgctcgtca
     2581 tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac
     2641 cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc
     2701 atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc
     2761 ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg
     2821 tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg
     2881 caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac
     2941 gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc
     3001 tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc
     3061 gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc
     3121 tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctgag
     3181 ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt gctggggcaa
     3241 cacgggggat ccactagttc catgagcgtc agaccccgta gaaaagatca aaggatcttc
     3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3421 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaggg
     3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4021 ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt acccggggat cctctagagt
     4081 cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt ggccgcagcc agctcgctga
     4141 gagccgtgaa cgacagggcg aacgccagcc cgccgacggc gagggttccg accgctgcaa
     4201 ctcccggtgc aaccttgtcc cggtctattc tcttcactgc accagctcca atctggtgtg
     4261 aatgcccctc gtctgttcgc gcaggcgggg ggctctattc gtttgtcagc atcgaaagta
     4321 gccagatcag ggatgcgttg caaccgcgta tgcccaggtc agaagagtcg cacaagagtt
     4381 gcagacccct ggaaagaaaa atggccagag ggcgaaaaca ccctctgacc agcggagcgg
     4441 gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg ggtgtctgcc gaccacatat
     4501 gggccggtca agataggttt ttaccccctc tcggctgcat cctctaagtg gaaagaaatt
     4561 gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag gagttgcaac ccggtagcct
     4621 tgttcacgac gagaggagac ctagttggca cgtcgcggat ggggatcgct gaagactcag
     4681 cgcagcggga ggatccaagc ctcatacgtc aacccgcagg acggtgtgag gtactacgcg
     4741 ctgcagacct acgacaacaa gatggacgcc gaagcctggc tcgcgggcga gaagcggctc
     4801 atcgagatgg agacctggac ccctccacag gaccgggcga agaaggcagc cgccagcgcc
     4861 atcacgctgg aggagtacac ccggaagtgg ctcgtggagc gcgacctcgc agacggcacc
     4921 agggatctgt acagcgggca cgcggagcgc cgcatctacc cggtgctagg tgaagtggcg
     4981 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
     5041 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
     5101 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
     5161 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
     5221 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
     5281 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
     5341 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
     5401 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
     5461 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
     5521 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
     5581 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
     5641 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
     5701 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
     5761 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
     5821 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
     5881 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
     5941 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     6001 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     6061 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     6121 ggtctctgac gagcgggaga actccctatc ag
//
