LOCUS       sequence-315737-        5417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0758:Dendra
FEATURES             Location/Qualifiers
     source          1..5417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1361..1384
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1641..2441
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2566..3154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3055..3074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgccgcgccc gctgcggggg ttacccaccg tcggcatcga
      121 cgacgtcgac gcgcatcacc gggtgatcgt gacgggatcc gcgggtgatc tggccgcggt
      181 gctgacgcgg ttgctgaagg ccgatcggct cgatgtcgag gtcgcgcacg cgcgcaccag
      241 ctggtcggcg cgtcgcgccc tgcgcggtac cgcccggcga gtgccgctga tccgcgacga
      301 gaccggaacc gtacttgttt ccgctgctga gtggcacggc aaggaggccg ccccgctcga
      361 gggcgaggcg atcgtcgacg acacggtgct gttcgacggc caggcgcacg gcgtgcggat
      421 cgagccgctc gacgcggtgc cgggcctgcg tgcgagcgtg ctgtcgcgcc ggggcaggcc
      481 gctgggctgg gtcagcgggc gcgcggctca actgggcacg ccgggcgccg tggtggtgcg
      541 cgacggcgcc acgatggcgc ggccggtacg ccgctcgacg ttctaccggc acacccaggg
      601 ctggctgcgc gtcggcaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
      661 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
      721 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
      781 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
      841 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
      901 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
      961 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
     1021 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
     1081 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
     1141 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     1201 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     1261 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     1321 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     1381 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     1441 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     1501 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     1561 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     1621 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     1681 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     1741 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     1801 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     1861 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     1921 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     1981 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     2041 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     2101 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     2161 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     2221 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     2281 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     2341 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     2401 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     2461 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     2521 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     3001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3181 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3241 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3301 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     3361 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     3421 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     3481 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     3541 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     3601 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     3661 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     3721 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     3781 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     3841 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     3901 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     3961 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     4021 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     4081 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     4141 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     4201 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     4261 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     4321 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     4381 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     4441 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     4501 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     4561 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     4621 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     4681 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc
     4741 gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag
     4801 tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc
     4861 gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt
     4921 ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac
     4981 gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc
     5041 cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg
     5101 cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc
     5161 cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt
     5221 gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
     5281 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
     5341 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
     5401 ggagaactcc ctatcag
//
