LOCUS       sequence-315744-        5297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0380:Dendra
FEATURES             Location/Qualifiers
     source          1..5297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1241..1264
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1521..2321
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2446..3034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2935..2954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaggcggct gtggattccg ctgttggtga ttgcggtggt
      121 cgccgttggc ggcttcactg tctcgcggtt gcacaacgtc ttcggcgccg agaagcgtcc
      181 gtcgtacgcc gatacgaagg ctgcggactc caaaccgttc gatcccaagc gcctgaccta
      241 cgaggttttc ggtcctcccg ggaccctggc cgacatcagc tacttcgacg aggactcgaa
      301 cccgcagttc gtagagaagg tcagtctgcc gtggtcgctg catttcgaca tcggtaagac
      361 caccgccgtc ggcagcatca tggcgcaggg tgacagcgac accatcggtt gccgcatcat
      421 cgtcgacgac gaagtcaagg ccgaaaaggt ctcgaaccag accaatgcct tcacctcctg
      481 cctcctgaag gccgcaaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
      541 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
      601 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
      661 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
      721 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
      781 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
      841 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
      901 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
      961 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
     1021 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     1081 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     1141 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     1201 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     1261 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     1321 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     1381 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     1441 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     1501 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     1561 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     1621 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     1681 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     1741 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     1801 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     1861 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     1921 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     1981 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     2041 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     2101 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     2161 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     2221 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     2281 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     2341 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     2401 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2461 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2521 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2581 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2641 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2821 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     2881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3061 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3181 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     3241 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     3301 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     3361 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     3421 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     3481 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     3541 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     3601 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     3661 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     3721 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     3781 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     3841 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     3901 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     3961 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     4021 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     4081 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     4141 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     4201 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     4261 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     4321 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     4381 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     4441 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     4501 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     4561 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc
     4621 gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag
     4681 tcgctgaagc gtggctacgc caagatcggt cggccggaac tccgcatcca cgacctccgc
     4741 gctgtcggcg ctacgttcgc cgctcaggca ggtgcgacga ccaaggagct gatggcccgt
     4801 ctcggtcaca cgactcctag gatggcgatg aagtaccaga tggcgtctga ggcccgcgac
     4861 gaggctatcg ctgaggcgat gtccaagctg gccaagacct cctgaaacgc aaaaagcccc
     4921 cctcccaagg acactgagtc ctaaagaggg gggtttcttg tcagtacgcg aagaaccacg
     4981 cctggccgcg agcgccagca ccgccgctct gtgcggagac ctgggcacca gccccgccgc
     5041 cgccaggagc attgccgttc ccgccagaaa tctagacggt gaccacaacg cgcccgcttt
     5101 gatcggggac gtcgaaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
     5161 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
     5221 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
     5281 ggagaactcc ctatcag
//
