LOCUS       sequence-315746-        5462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0004:Dendra
FEATURES             Location/Qualifiers
     source          1..5462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1406..1429
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1686..2486
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2611..3199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3100..3119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgaccgggcc cttcgacgac gacggtcccg aggaggacgc
      121 gccggtcccc gctcccccgg atcatctggc cggtctgcgc ggcatcgatc tggtgcgtcg
      181 cacgctcgag gaggcccgcg gcgcggcacg cagccagggc aaggatgtcg gccgcggccg
      241 cagcggcccg gcccgccgag ttggcgggaa ccgcaggcgc agaacctggt cggggccggg
      301 acccgatgcg cgggacccgc aactactcgg tgcggtcacc caggacctcg cgaaatcccg
      361 cgggtggagc gcacgcgtcg ccgaaggctc ggtgatcggc cggtggcgtg cggtggtcgg
      421 cgaccagatc gccgatcacg cgaccccgac ggcgctcaac gaaggcgtgc tgaccgtgac
      481 ggccgagtcg acggcgtggg ccacgcagct gcggatggtg cagtcacaac tgctggccaa
      541 gatcgccgcg gtggtcggcg acggtgtggt gaccacgttg aagatcgtcg gccccgcggg
      601 tccgtcgtgg cgcaagggcc gctaccacgt ctcgggacgc gggccccgcg acacctacgg
      661 gaagcttggt gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg
      721 tgtcaaggtg catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg
      781 taagggtaag ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc
      841 tctcccattc tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac
      901 caagtacccc gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg
      961 ggagcggacg atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct
     1021 ggaaggggac tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg
     1081 cccggtcatg cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg
     1141 ggacggcctc ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta
     1201 cctatgcgac ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca
     1261 cttcgtggac caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt
     1321 gtacgagcac gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg
     1381 cggcggggag aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata
     1441 accccttggg gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa
     1501 ttcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat
     1561 gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag
     1621 gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc
     1681 gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc
     1741 tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct
     1801 ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg
     1861 ctacgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc
     1921 cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct
     1981 ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac
     2041 aggcagagca gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg
     2101 tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca
     2161 acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact
     2221 gcaccattct tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct
     2281 gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc
     2341 cagtcggtca tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg
     2401 gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat
     2461 gcgatgccgc tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga
     2521 ggggcaaggt cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc
     2581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3001 gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg
     3061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     3361 cggccgcggt acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg
     3421 ccaccccgtt ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc
     3481 cgccgacggc gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc
     3541 tcttcactgc accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg
     3601 ggctctattc gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta
     3661 tgcccaggtc agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag
     3721 ggcgaaaaca ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt
     3781 tggaagaatg ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc
     3841 tcggctgcat cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag
     3901 agttgcacag gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca
     3961 cgtcgcggat ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc
     4021 aacccgcagg acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc
     4081 gaagcctggc tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag
     4141 gaccgggcga agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg
     4201 ctcgtggagc gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc
     4261 cgcatctacc cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt
     4321 gcgtggtggg ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc
     4381 ctccgggcgg tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg
     4441 atcgagcaga aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac
     4501 atcgtcgccg ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg
     4561 agcctccggt tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg
     4621 acgatgaagc tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc
     4681 aacgccaaga ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg
     4741 atccgagcgc acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg
     4801 accacgacgc agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc
     4861 tacgccaaga tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg
     4921 ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact
     4981 cctaggatgg cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag
     5041 gcgatgtcca agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact
     5101 gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc
     5161 cagcaccgcc gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc
     5221 cgttcccgcc agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
     5281 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
     5341 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
     5401 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
     5461 ag
//
