LOCUS       sequence-31576-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-MDR2-NBD
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcttagaat ctttttctct tacatctcat
      121 gaaaaaaaat ttggtgtaaa tatcgaattt tcagatgtaa attttagtta tccaaaacaa
      181 acaaatcata gaacgttaaa atctataaat ttttttattc catctggtac aacctgtgca
      241 ttagttggac atacgggttc aggaaaatca accattgcaa aattattata tcgtttttat
      301 gatgctgaag gagatattaa aataggaggt aaaaatgtta acaaatataa tcgaaattct
      361 attcgaagta ttattggtat tgtacctcaa gatacaatat tatttaatga aacaataaaa
      421 tataatatat tatatggtaa attagatgca acagatgagg aagtaattaa agctacaaaa
      481 tcagctcagt tatatgattt tatagaagca cttccgaaaa aatgggatac aattgttgga
      541 aataaaggta tgaaattatc aggtggtgaa agacaaagaa ttgctattgc tagatgttta
      601 ttaaaagatc ctaaaattgt tatttttgat gaagctacaa gttctttaga ttctaaaaca
      661 gaatatttat ttcaaaaagc tgttgaagat ttaagaaaaa atagaactct cataattatt
      721 gctcatagac tcagtactat atcttcagca gaatcaatta ttttactaaa taaaggaaaa
      781 attgtagaaa agggaactca taaagattta cttaaactta atggtgagta tgcagaaatg
      841 tggaatatgc aatct
//
