LOCUS       sequence-315767-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS
  TITLE     Optimization of S. aureus dCas9 and CRISPRi Elements for a Single 
            Adeno-Associated Virus that Targets an Endogenous Gene.
  JOURNAL   Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 
            10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11.
  PUBMED    33024795
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2020-09-6"; volume: "19:139-148. doi"; 
            pages: " 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11"
COMMENT     Sequence Label: (364) SB KRAB-SadCas9
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        295..607
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    303..321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    339..357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    446..464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    481..499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     580..604
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             694..879
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             4132..4158
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    4202..4323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4213..4232)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4267..4286
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             join(4991..5608,5609..5614,5615..5650,5651..5653,5654..5689,
                     5690..5692,5693..5728,5729..5734)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5741..5797
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5792..6592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5852..5871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6462..6481
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6647..6664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6653..6877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7203..7220)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7374..7962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7454..7473)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8133..8924,8925..8993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8756..8775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8994..9098)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agacgagttt
      301 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      421 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      481 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      541 tgtcgattag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      601 gatcgcctgg agccaattcc aactcttttg ccttatacct actttccgta ccacttccta
      661 ccctcgaaag gcctctgaga attcgccacc atgcggacac tagtgacctt caaggatgta
      721 tttgtggact tcaccaggga ggagtggaag ctgctggaca ctgctcagca gatcgtgtac
      781 agaaatgtga tgctggagaa ctataagaac ctggtttcct tgggttatca gcttactaag
      841 ccagatgtga tcctccggtt ggagaaggga gaagagcccg gcggttccgg cggagggtcc
      901 atgggcaaac ggaactacat cctggggctt gccattggga taaccagcgt tggctacgga
      961 attattgatt atgagacacg cgatgtgatt gacgccgggg ttaggctgtt caaagaggcc
     1021 aacgttgaaa acaacgaggg aagacggagt aagcgcggag caagaagact caagcgcaga
     1081 cggagacatc ggattcagag ggtgaaaaag ctgctcttcg attacaatct cctgaccgat
     1141 catagtgagc tgagcggaat caacccctac gaggcgcgag tgaaagggct ttcccagaag
     1201 ctgtccgaag aggagttctc cgccgcgttg ctgcacctgg ccaaacggag gggggttcac
     1261 aatgtaaacg aagtggagga ggacacgggc aatgaactta gtacgaaaga acagatcagt
     1321 aggaactcta aggctctcga agagaaatac gtcgctgagt tgcagcttga gagactgaaa
     1381 aaagacggcg aagtacgcgg atctattaat aggttcaaga cttcagatta cgtaaaggaa
     1441 gccaagcagc tcctgaaagt acagaaagcg taccatcagc tcgatcagag cttcatcgat
     1501 acctacatag atttgctgga gacacggagg acatactacg agggcccagg ggaaggatct
     1561 ccttttgggt ggaaggacat caaggaatgg tacgagatgc ttatgggaca ttgtacatat
     1621 tttccggagg agctcaggag cgtcaagtac gcctacaatg ccgacctgta caatgccctc
     1681 aatgacctca ataacctcgt gattaccagg gacgagaacg agaagctgga gtactatgaa
     1741 aagttccaga ttatcgagaa tgtgtttaag cagaagaaga agccgacact taagcagatt
     1801 gcaaaggaaa tcctcgtgaa tgaggaagat atcaagggat acagagtgac aagtacaggc
     1861 aagcccgagt tcacaaatct gaaggtgtac cacgatatta aggacataac cgcacgaaag
     1921 gagataatcg aaaacgctga gctcctcgat cagatcgcaa aaattcttac catctaccag
     1981 tctagtgagg acattcagga ggaactgact aatctgaaca gtgagctcac ccaagaggaa
     2041 attgagcaga tttcaaacct gaaaggctac accgggacgc acaatctgag cctcaaagca
     2101 atcaacctca ttctggatga actttggcac acaaatgaca accaaattgc catattcaac
     2161 cgcctgaaac tggtgccaaa aaaagtggat ctgtcacagc aaaaggaaat ccctacaacc
     2221 ttggttgacg attttattct gtcccccgtt gtcaagcgga gcttcatcca gtcaatcaag
     2281 gtgatcaatg ccatcattaa aaaatacgga ttgccaaacg atataattat cgagcttgca
     2341 cgagagaaga actcaaagga cgcccagaag atgattaacg aaatgcagaa gcgcaaccgc
     2401 cagacaaacg aacgcataga ggaaattata agaacaaccg gcaaagagaa tgccaagtat
     2461 ctgatcgaga aaatcaagct gcacgacatg caagaaggca agtgcctgta ctctctggaa
     2521 gctatcccac tcgaagatct gctgaataat ccattcaatt acgaggtgga cgccatcatc
     2581 cctagatccg taagctttga caattccttc aataacaaag ttctggttaa acaggaggaa
     2641 aattctaaaa aagggaaccg gaccccgttc cagtacctga gctccagtga cagcaagatt
     2701 agctacgaga cttttaagaa acatattctg aatctggcca aaggcaaagg caggatcagc
     2761 aagaccaaga aggagtacct cctcgaagaa cgcgacatta acagatttag tgtgcagaaa
     2821 gatttcatca accgaaacct tgtcgatact cggtacgcca cgagaggcct gatgaatctc
     2881 ctcaggagct acttccgcgt caataatctg gacgttaaag tcaagagcat aaatggggga
     2941 ttcaccagct ttctgaggag aaagtggaag tttaagaagg aacgaaacaa aggatacaag
     3001 caccatgctg aggatgcttt gatcatcgct aacgcggact ttatctttaa ggaatggaaa
     3061 aagctggata aggcaaagaa agtgatggaa aaccagatgt tcgaggagaa gcaggcagag
     3121 tcaatgcctg agatcgagac agagcaggaa tacaaggaaa ttttcatcac ccctcatcag
     3181 attaaacaca taaaggactt caaagactat aaatactctc atagggtgga caaaaaaccc
     3241 aatcgcgagc tcattaatga caccctgtac tcaacacgga aggatgataa aggtaatacc
     3301 ttgattgtga ataatcttaa tggattgtat gacaaagata acgacaagct caagaagctg
     3361 atcaacaagt ctccagagaa gctccttatg tatcaccacg acccacagac ttatcagaaa
     3421 ttgaaactga tcatggagca atacggggat gagaagaacc cactctacaa atattatgag
     3481 gaaacaggta attacctgac caagtactcc aagaaggata acggaccagt gatcaaaaag
     3541 ataaagtact atggcaacaa acttaatgcg catttggaca taactgacga ttaccccaat
     3601 tctcgaaaca aggttgtgaa gctctccctg aagccttata gatttgacgt gtacctggat
     3661 aatggggttt ataaattcgt caccgtgaaa aatctggacg tgatcaaaaa ggagaactat
     3721 tatgaagtaa actcaaagtg ctatgaggag gcgaagaagc tgaagaagat ctccaatcag
     3781 gccgagttca tcgcttcctt ctataataac gatctcatca agatcaatgg agagctttat
     3841 cgcgtcattg gtgtgaacaa tgacttgctg aacaggatcg aagtcaatat gatagacatt
     3901 acctaccggg agtatctcga aaacatgaat gataaacggc cgcctcgcat catcaagaca
     3961 atcgcatcta aaactcagtc aataaaaaag tactctaccg atatcctggg gaatctctat
     4021 gaagtgaagt caaagaagca cccacaaatc attaaaaaag gtggatccgc agaagcagca
     4081 gcaaaggaag cagcagcaaa ggaagcagca gcaaaggcag tcgacaccgg tccagcagct
     4141 aagagagtta aactagatta gcggccgcat cgtcaggcca agcttccatc gatagacatg
     4201 ataagataca ttgatgagtt tggacaaacc acaacaagaa tgcagtgaaa aaaatgcttt
     4261 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4321 gttaacaaca acaattgcat tcattttatg tttcaggtac ctaggcgaga ccctgtctca
     4381 caaaataaag taagcccgga ctgagtgcgg aaaggcgggc ctggcgggtc tggtctcccc
     4441 atgcgggcca ccagaggccc tgcagccttc agtcgcttga aggggtaatg gcgcttccac
     4501 tcacaaacat ggcggacaga gcgtgtgaac gagatgaaca gcccctcaaa aatatggccg
     4561 ccgaggctgg acggccgtgc cccagcagca ccgcctccgc gccccacgtg atctctcgcc
     4621 gggcacagcg ctgaccgcgg aggtccaacc ggaagaatgt ccggattgga cattcggaag
     4681 agggcccgcc ttccctgggg aatctctgcg cacgcgcaga acgcttcgac caatgaaaac
     4741 acaggaagcc gtccgcgcaa ccgcgttgcg tcacttctgc cgcccctgtt tcaaggtata
     4801 tagccgtaga cggaacttcg cctttctctc ggccttagcg ccattttttt gggtgagtgt
     4861 tttttggttc ctgcgttggg attccgtgta caatccatag acatctgacc tcggcactta
     4921 gcatcatcac agcaaactaa ctgtagcctt tctctctttc cctgtagaaa cctctgcacc
     4981 tgaggccacc atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact
     5041 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     5101 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     5161 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     5221 ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc
     5281 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     5341 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     5401 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     5461 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     5521 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     5581 ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt
     5641 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     5701 tcttgacgat tttgaccttg acatgctccc cgggtccggc gctactaact tcagcctgct
     5761 gaagcaggct ggtgacgtcg aggagaatcc tggtcccatg attgaacaag atggattgca
     5821 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5881 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5941 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6001 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6061 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6121 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6181 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6241 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6301 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6361 cggtgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6421 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6481 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6541 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gattcgaagg
     6601 cctgtcgtga agcttgggga tcaattctct agagctcgct gatcagcctc gactgtgcct
     6661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6901 tgggcctact agctactcgg gaccccttac cgaaacatcg ccgcattctg cagaggagtc
     6961 gagtgtatgt aaacttctga cccactggga atgtgatgaa agaaataaaa gctgaaatga
     7021 atcattctct ctactattat tctgatattt cacattctta aaataaagtg gtgatcctaa
     7081 ctgacctaag acagggaatt tttactagga ttaaatgtca ggaattgtga aaaagtgagt
     7141 ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtata gggatccgct
     7201 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7261 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7321 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7381 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7441 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7501 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7561 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7621 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7681 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7741 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7801 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7861 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7921 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7981 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8041 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8101 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8161 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8221 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8281 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8341 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8401 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8461 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8521 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8581 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8641 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8701 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8761 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8821 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8881 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8941 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9001 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9061 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9121 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     9181 gtaagcgtta atattttgtt aaaatt
//
