LOCUS       sequence-31577-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-ClpP
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(811..829)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      825..872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt
       61 gtagatctgg gtaccgagaa cctgtacttc caatccatgg atattaaaga catgaaaaaa
      121 gatgtcaaat tatttttttt taaaaaaaga attatatatt taacagatga aataaataaa
      181 aaaacagcag atgaacttat tagtcaatta ttatatttag ataatataaa tcataatgat
      241 attaaaatat atattaattc accaggaggt tccataaatg agggactagc cattctagat
      301 atatttaatt atattaaatc agatatacaa acaatatcat ttggtttagt tgcatctatg
      361 gcatcagtga ttttagctag tggaaaaaaa ggaaaaagaa agtctttacc taattgtaga
      421 atcatgatac atcaacctct tggtaacgct tttggtcatc cacaagatat tgaaatacaa
      481 acaaaagaaa tcctttatct taaaaaactt ctatatcatt atctatcatc atttaccaat
      541 cagacagtcg aaacgattga aaaggattct gatagagatt attatatgaa tgcactagaa
      601 gcaaaacaat atggaatcat agatgaagtt attgaaacta aattaccaca tccatatttt
      661 aataaagtag aaaaataaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      721 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      841 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      901 cgaatggga
//
