LOCUS       sequence-31578-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pv-UBC
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(546..564)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      560..607
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(782..787,788..804,805..810))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(837..855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gcaaatacaa tatggggaac gctaactaca
      121 gaatacaaaa ggaattacat aactttctga ataacccccc tattaactgc actttagatg
      181 tacacccaaa taatatccga atatggattg tgaaatatgt agggctggaa aatactatat
      241 acgctaatga agtttacaaa ctgaagataa tatttccaga tgattatcct ttgaagcccc
      301 ccattgtgta ttttttacag aagccaccta aacataccca cgtatattcc aatggagaca
      361 tatgcttaag tcttctcggt gatgactaca accctagttt gtccatttcc ggcttagtcc
      421 tctccatcat atcgatgtta tcttctgcta aggaaaaaaa attgccctga tgacgaagct
      481 tgcggccgca ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      541 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      601 ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc
      661 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
      721 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
      781 cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
      841 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtc
//
