LOCUS       sequence-31579-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-ACPS
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(653..671)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      667..714
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(889..894,895..911,912..917))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg ccatcatata ataggtattg gtacagatat
      121 tttatgtgtt aatagaattt ataaaattct tgaaaaaaat ataaatttta ttaaaaaagt
      181 tttaaatcca tttgaattag ctgaatttga aacacaaaaa aaaaaattaa acgaaaaaat
      241 aaataaaagt aatgaattaa aaaaattagc aatttatgtt agtaaaaaat ttgcagcaaa
      301 agaagctata cttaaatcta tgggtcgtgg attaagttct atatctaaat atggattaag
      361 tatgaatgat attgaaataa agaatgataa atatggaaaa cctcatgttt atttatatgg
      421 aaaagctaaa aaggtagctt acgaaatggg tatagtcaaa atatttttat caattagtga
      481 tgagaaaatt ataaatagcc agacaaataa tatttcgtcc aattttccga catttattat
      541 acaagcacag gctctagctg taggctcaaa tgtatgatga cgaagcttgc ggccgcactc
      601 gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      661 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      721 ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata accaagccta
      781 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
      841 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
      901 cgatgataag ctgtcaaaca tgagaattc
//
