LOCUS       sequence-31580-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-UBC
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(583..601)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      597..644
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(819..824,825..841,842..847))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(874..892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcgaat tgaagccttc aagaacggta
      121 gaaaaacata taaaaacaaa atataattta ggaaatgcaa attatagaat tcaaaaagag
      181 ttaaataatt ttttaaagaa tccaccaatt aattgtacta ttgatgtaca tccaagtaat
      241 attagaatat ggattgtaca atatgtaggt ttagagaata ctatatatgc taacgaagtt
      301 tataaaatta aaataatttt tccggataat tatcctttaa agccacctat tgtatatttt
      361 ttacaaaaac ctcctaaaca tacacatgta tattctaatg gagatatttg tttaagtgta
      421 ttaggtgatg attataatcc aagcctctct atttctggat taattttatc tatcatatca
      481 atgttatcat ctgcaaaaga aaaatgatga cgaagcttgc ggccgcactc gaggatccgg
      541 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      601 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      661 tatccggata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
      721 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
      781 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag
      841 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
      901 taatgtcatg ataataatgg t
//
