LOCUS       sequence-31581-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-FDPS
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc agagaaaact tgtatttcca gggcgaatat gattatacgg attttattaa
      121 ttattacgat aaatttaaag taatagtata taatgtttta aaaaaattac cattaaatga
      181 tgaaataaga aaaccagtaa tcgaatatta cttaaactgt attgattata acgtaaaaaa
      241 aggcaagcat attcgtggca aaattttagt gttaatctca agtttaagtt cagcatattc
      301 aaatataaag agagactcca tttatttgtt agggtgggtg gttgaagcaa tacaagcatt
      361 gatattaatt gcggacgata ttatggattc tggtaaattt agaagaggag cgccttgttg
      421 gtatattgtt catggtcaat cgaatgcaat taatgacatt tttttcttga aaatgctatc
      481 actttcactg atctttgagc tttctagtgt attcggaaat gatattgtta tgaaaatcca
      541 gaaaatatac aatgaatcta ttttttttac ggtattgggc caacatttag atctgtcata
      601 ttttgattta tctaaagctg acaaaatatc cgaaagatac ttttcaatgg tagaaatgaa
      661 gacttctcgc tatacgtttt atatgcctgt attttttggc ttaacacttt ctgaaattca
      721 agtttcttca gcgcaattaa acttgattga agctatcctc tataaactgg gcgaattcta
      781 tcaagtgcac aatgatgttt ctgactactt atttaatgat agcaatgcag atgatatttg
      841 caggtttaaa cttacatggc cactacaaaa atcgtttgaa attgctgatg a
//
