LOCUS       sequence-315824-        1734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CheRiff-eGFP channelrhodopsin (plasma membrane).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS010: CheRiff-eGFP channelrhodopsin (plasma...
FEATURES             Location/Qualifiers
     source          1..1734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..948
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(1018..1020,1021..1734)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1018..1020,1021..1731)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1060..1081)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1321..1340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1668..1689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggcggag ctcctgctcc agacgctcac agcgccccac ctggaaacga ttctgccgga
       61 ggcagtgagt accatgcccc agctggatat caagtgaatc caccctacca ccccgtgcat
      121 gggtatgagg aacagtgcag ctccatctac atctactatg gggccctgtg ggagcaggaa
      181 acagctaggg gcttccagtg gtttgccgtg ttcctgtctg ccctgtttct ggctttctac
      241 ggctggcacg cctataaggc cagcgtggga tgggaggaag tgtacgtgtg ctccgtggag
      301 ctgatcaaag tgattctgga gatctatttc gagttcacca gtcctgctat gctgttcctg
      361 tacggaggga acattacccc atggctgaga tatgccgaat ggctgctgac atgtcccgtg
      421 atcctgattc atctgtctaa catcaccggc ctgagtgagg catacaataa gcggacaatg
      481 gctctgctgg tgtccgacct gggaactatt tgcatgggag tgacagccgc tctggccact
      541 gggtgggtga agtggctgtt ttactgtatc ggcctggtgt atggaaccca gacattctac
      601 aacgctggaa tcatctacgt ggagtcttac tatatcatgc ctgccggcgg ctgtaagaaa
      661 ctggtgctgg ccatgactgc cgtgtactat tctagttggc tgatgtttcc cggcctgttc
      721 atctttgggc ctgaaggcat gcacaccctg agcgtggctg ggtccactat tggccatacc
      781 atcgccgacc tgctgtccaa gaatatttgg ggactgctgg ggcacttcct gcggatcaaa
      841 attcacgagc atatcattat gtacggcgat atcaggagac cagtgagctc ccagtttctg
      901 ggacgcaagg tggacgtgct ggccttcgtg acagaggaag ataaagtggc ggccgccaag
      961 agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat caacgtggtg
     1021 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1081 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1141 ctgaccctga agttcatttg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1201 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1321 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1441 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1501 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1621 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1681 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaa
//
